Anti-Phospho-PPP1CA (T320) Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P62136

Core Product Specifications and Parameters

Biological Background: PPP1CA Function and Localization

• Protein Identity and Holoenzyme Formation: Serine/threonine-protein phosphatase PP1-alpha catalytic subunit that associates with over 200 regulatory proteins to form substrate-specific holoenzymes, dephosphorylating hundreds of biological targets (PubMed:28216226, 30158517, 35768504).
  Related references: PMID:28216226, PMID:30158517, PMID:35768504

• Core Cellular Functions: Essential for cell division, transcription elongation, glycogen metabolism, muscle contractility, and protein synthesis (PubMed:35768504, 35830882, 35831509). Also regulates ionic conductances, long-term synaptic plasticity, and postsynaptic CaMKII dephosphorylation.
  Related references: PMID:35768504, PMID:35830882, PMID:35831509

• Transcription Regulation via PNUTS-PP1 Complex: Catalytic component of the PNUTS-PP1 complex that mediates RNA polymerase II pause-release and termination by dephosphorylating factors like AFF4, CDK9, SUPT5H (PubMed:39603239, 39603240, 31677974). Also promotes RNAPII degradation upon replication stress and regulates chromatin structure during mitosis-interphase transition (PubMed:33264625, 20516061).
  Related references: PMID:39603239, PMID:39603240, PMID:31677974, PMID:33264625, PMID:20516061

• Subcellular Localization: Found in the cytoplasm, nucleus (nucleoplasm and nucleolus), and at the postsynaptic density, consistent with its diverse regulatory roles.

• Post-translational Modifications and Metal Binding: A phosphoprotein and acetylated protein that requires manganese (Mn²⁺) as a cofactor for its hydrolase activity.

• Key Signaling Pathway Involvement: Acts as a core phosphatase in the SHOC2-MRAS-PP1c (SMP) complex to dephosphorylate and activate RAF kinases, stimulating the MAPK pathway (PubMed:35768504, 35830882, 35831509, 36175670). Also dephosphorylates FOXP3 in Treg cells, CENPA, ATG16L1, and innate immune sensors IFIH1/MDA5 and RIG-I (PubMed:23396208, 25556658, 26083323, 23499489).
  Related references: PMID:35768504, PMID:35830882, PMID:35831509, PMID:36175670, PMID:23396208, PMID:25556658, PMID:26083323, PMID:23499489

• Additional Biological Roles: Regulates circadian rhythm by balancing CSNK1D/E activity and PER phosphorylation (PubMed:21712997). Also involved in embryonic development (neural tube closure, optic fissure closure, ENCC migration; By similarity) and necessary for alphavirus replication.
  Related references: PMID:21712997

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphorylated peptide around Thr320 of human PPP1CA; specific detection of phosphorylated PPP1CA at this site requires phosphorylation to be maintained during sample preparation. Consider including phosphatase inhibitors in lysis buffers.
• For Western blot, the predicted molecular weight is approximately 38 kDa; a band near this size is expected in relevant samples. Non-specific bands may appear depending on sample type and antibody concentration; optimization of primary antibody dilution is recommended.
• Cross-reactivity with mouse and rat is expected based on sequence conservation; however, validation in the target species and tissue is advised prior to quantitative studies.
• For IHC-P, antigen retrieval conditions and antibody dilution should be optimized per tissue type. For ELISA, consider using a non-phospho peptide control to confirm specificity.

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At CamelBio, we provide diagnostic manufacturers, laboratories, and research institutes with a complete sourcing solution for IVD raw materials—supporting every phase from concept to clinic. Our catalog includes high-quality antibodies like this phospho-specific anti-PPP1CA (T320) antibody, tailored for phosphatase signaling and cell cycle research, along with validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and rare-target raw material procurement. Partner with CamelBio to streamline your assay development and secure the critical components you need.