Anti-Phospho-POLR2A CTD-S7 Rabbit pAb for WB, IHC-P, ELISA, ChIP - P24928

Core Product Specifications and Parameters

Biological Background: POLR2A Function and Localization

• POLR2A, also known as RPB1 or POLR2, is the largest subunit of RNA polymerase II (Pol II), a DNA-dependent RNA polymerase responsible for synthesizing mRNA precursors and many functional non-coding RNAs.
• It forms the catalytic core together with the second largest subunit POLR2B, using a Mg²⁺-coordinating DxDGD motif for nucleotide polymerization and a 3'→5' exonuclease activity for proofreading. Related references: PMID:23748380, PMID:27193682, PMID:30190596, PMID:8381534, PMID:9852112
• The unique C-terminal domain (CTD) consists of 52 tandem heptapeptide repeats (YSPTSPS) and serves as a platform for transcriptional regulators. Phosphorylation at Ser5 facilitates promoter escape, whereas Ser2 phosphorylation is required for pause release and elongation. Related references: PMID:24207025, PMID:26124092
• CTD lysine acetylation activates growth-factor-induced immediate early genes, while methylation and dimethylation repress target gene expression, fine-tuning transcriptional output. Related references: PMID:24207025, PMID:26124092
• POLR2A primarily localizes to the nucleus but is also detected in the cytoplasm and on chromosomes, consistent with its dynamic role in transcription.
• It acts as an RNA-dependent RNA polymerase that extends non-coding RNAs and can function as a replicase for Hepatitis delta virus.
• Keywords associated with POLR2A include phosphoprotein, acetylation, methylation, DNA-binding, metal-binding, transcription, host-virus interaction, and disease variant, reflecting its complex regulation.
• Mutations in POLR2A are linked to neurodevelopmental disorders such as intellectual disability (NEDHIB).

Experimental Guidance and Technical Tips

• For western blot, use a low-percentage SDS-PAGE gel (e.g., 6% or gradient) and wet transfer to ensure efficient transfer of the 217 kDa protein.
• Include phosphatase inhibitor cocktails in lysis buffers to preserve the S7 phosphorylation signal; consider using a non-phospho peptide or phosphatase-treated sample as a negative control.
• In IHC-P, optimize antigen retrieval conditions (citrate or EDTA-based buffers) and use a polymer detection system to amplify signal.
• For ELISA, employ the phospho-peptide as a positive control and the corresponding non-phospho peptide to confirm specificity.
• In ChIP, cross-link proteins to DNA and shear chromatin to 200–500 bp fragments; the antibody may enrich Pol II at promoters of actively transcribed genes.
• Validate antibody performance in the relevant species and tissue context before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a one-stop platform for IVD raw materials, supporting projects from concept to clinic. This anti-Phospho-POLR2A CTD-S7 polyclonal antibody addresses demand for transcription research reagents, and our portfolio extends to validated antibody pairs, optimized monoclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets. Partner with CamelBio to streamline your procurement and accelerate assay development.