Anti-Phospho-PLCG2 (Y759) Rabbit Polyclonal Antibody for WB, ELISA - P16885

Core Product Specifications and Parameters

Biological Background: PLCG2 Function and Localization

• PLCG2 (1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2) is a member of the phosphoinositide-specific phospholipase C family that hydrolyzes PIP2.
• It plays a crucial role in transmembrane signaling by generating the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). Related references: PMID:19394299, PMID:23000145
• The enzyme localizes to membrane rafts, which are specialized microdomains facilitating signal transduction.
• PLCG2 is a phosphoprotein, and its activity is regulated by phosphorylation at specific tyrosine residues, including Y759.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y759 of human PLCG2. For western blot analysis, include phosphatase inhibitors in the lysis buffer to preserve the phosphorylation signal.
• Validate the antibody's specificity by treating samples with phosphatase or using non-phosphorylated peptide blocking controls in both WB and ELISA.
• PLCG2 has a molecular weight of ~148 kDa; ensure adequate separation on SDS-PAGE and transfer conditions for high-molecular-weight proteins.
• Cross-reactivity has been confirmed for human; if applying to other species, test a pilot experiment first.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting your diagnostic development from concept to clinic. This phospho-PLCG2 antibody is part of our curated selection for signal transduction research. We supply validated antibody pairs, optimized recombinant proteins, and ancillary reagents, along with sourcing assistance for rare targets to streamline your assay development.