Anti-Phospho-PLCG2 (Y1217) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P16885

Core Product Specifications and Parameters

Biological Background: PLC gamma 2 (PLCG2) Function and Localization

• PLCG2 encodes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2, also known as Phosphoinositide phospholipase C-gamma-2, Phospholipase C-IV (PLC-IV), and Phospholipase C-gamma-2 (PLC-gamma-2).
• It catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), playing a crucial role in transmembrane signaling. Related references: PMID:19394299 PMID:23000145
• The protein contains SH2 and SH3 domains, as well as repeat regions, indicating its role in signal transduction and protein interactions.
• PLCG2 is associated with membrane rafts, where it likely participates in localized signaling events.
• Phosphorylation is a key regulatory modification of PLCG2, consistent with its classification as a phosphoprotein.
• Variants in PLCG2 are associated with human diseases, including familial cold autoinflammatory syndrome 3 (FCAS3) and autoinflammation and PLCG2-associated antibody deficiency and immune dysregulation (APLAID).
• Additional keywords from UniProt include 3D-structure, calcium, hydrolase, lipid degradation, lipid metabolism, and proteomics identification, reflecting its diverse functional roles.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphorylated peptide corresponding to Y1217 of human PLCG2; thus, it is specific for the phosphorylated form at that site. Verify phosphorylation status in your experimental system using appropriate phosphatase controls.
• For Western Blot, the predicted molecular weight of PLCG2 is 148 kDa. Optimize blotting conditions for high-molecular-weight proteins.
• In immunofluorescence/immunocytochemistry (IF/ICC), membrane raft localization may be observed; include appropriate permeabilization and confirm colocalization with raft markers if needed.
• For ELISA, ensure peptide coating and antibody dilution are optimized for phospho-specific detection.
• As a rabbit monoclonal antibody, it offers advantages in specificity and reproducibility; however, validate in your specific sample type and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and researchers with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. For targets like PLCG2, essential in signal transduction and immune signaling, we offer reliable sourcing solutions from discovery to production scale. Whether you need phospho-specific antibodies for kinase pathway studies or rare target raw materials, CamelBio serves as your one-stop sourcing bridge from concept to clinic.