Anti-Phospho-PLC gamma 2 (PLCG2)-Y759 Monoclonal Antibody for WB, IF/ICC, ELISA - P16885

Core Product Specifications and Parameters

Biological Background: PLC gamma 2 Function and Localization

• PLCG2 encodes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-2 (PLC-gamma-2), a multidomain enzyme containing SH2 and SH3 domains that functions as a key signal transducer.
• It catalyzes the hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2) to produce the second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), mediating crucial transmembrane signaling. Related references: PMID:19394299 | PMID:23000145
• PLC-gamma-2 is localized to membrane rafts, where it participates in receptor-mediated signal transduction and immune cell activation.
• Phosphorylation at tyrosine 759 is a critical regulatory event for PLC-gamma-2 activity, often downstream of immune receptors and kinases.
• Genetic variants in PLCG2 are associated with autoinflammatory diseases, including familial cold autoinflammatory syndrome 3 (FCAS3) and APLAID (autoinflammation, PLCG2-associated antibody deficiency and immune dysregulation).
• Additional keywords include lipid degradation, calcium signaling, and phosphoprotein, reflecting its central role in cellular lipid metabolism and signal relay.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y759; therefore, the antibody specifically detects PLC-gamma-2 phosphorylated at this site.
• For Western blot, the predicted molecular weight is ~148 kDa. Use fresh samples with phosphatase inhibitors to preserve phosphorylation status.
• In immunofluorescence/immunocytochemistry, consider permeabilization and fixation conditions that preserve membrane raft localization.
• ELISA applications may benefit from using a phospho-peptide blocking control to confirm specificity.
• Validate the antibody in relevant human cell lines or tissues where PLCG2 is expressed and phosphorylated.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, including this phospho-specific PLCG2 antibody targeting a key signaling enzyme relevant to immunology and cancer research. Our comprehensive supply portfolio covers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, enabling you to accelerate assay development from concept to clinic. Partner with CamelBio for reliable, science-backed reagents and expert technical support.