Anti-Phospho-PLC gamma 1 (PLCG1)-Y783 Rabbit Monoclonal Antibody for WB - P19174

Core Product Specifications and Parameters

Biological Background: PLC-gamma-1 Function and Localization

• PLCG1 encodes 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1, also designated PLC-gamma-1, PLC-II, PLC-148, and phosphoinositide phospholipase C-gamma-1.
• The enzyme hydrolyzes PIP2 to generate second messengers diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), triggering downstream calcium and PKC signaling.
• PLC-gamma-1 is activated downstream of receptor tyrosine kinases including PDGFRA/B, EGFR, and FGFRs, linking growth factor stimulation to intracellular pathways.
• It contributes to actin reorganization and cell migration. Related references: PMID:17229814
• As a guanine nucleotide exchange factor (GEF), PLC-gamma-1 binds DNM1 and catalyzes GDP dissociation, enhancing dynamin-dependent endocytosis.
• Subcellularly, it localizes to lamellipodia and membrane ruffles at the leading edge of migrating cells.
• The protein features SH2 and SH3 domains, and is regulated by phosphorylation, acetylation, and ubiquitination.
• Alternative splicing produces multiple isoforms, and mutations are implicated in disease.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide specific to the phosphorylated Tyr783 residue. To preserve phosphorylation, ensure lysis buffers contain phosphatase inhibitors.
• For signal induction, treat cells with appropriate growth factors (e.g., EGF, PDGF) to stimulate PLC-gamma-1 Y783 phosphorylation prior to harvesting.
• Predicted mouse cross-reactivity enables validation in murine models; however, verify performance in the relevant sample system.
• Include a non-phosphopeptide blocking control or phosphatase treatment to confirm phospho-specificity in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this phospho-specific PLC-gamma-1 antibody as part of a broad portfolio of IVD raw materials for signal transduction and oncology research. We offer one-stop access to validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets, supporting diagnostic manufacturers and research labs from concept to clinic.