Anti-Phospho-PLA2G4A-S505 Rabbit Polyclonal Antibody for WB, ELISA - P47712

Core Product Specifications and Parameters

Biological Background: Cytosolic Phospholipase A2 (PLA2G4A) Function and Localization

• Cytosolic phospholipase A2 (cPLA2α), encoded by the PLA2G4A gene, is a key enzyme in eicosanoid and inflammatory mediator biosynthesis. It selectively hydrolyzes the sn-2 arachidonoyl group from membrane phospholipids, supplying arachidonic acid for the cyclooxygenase pathway (PubMed:10358058, PubMed:17472963, PubMed:18451993).
  Related references:
  PMID:10358058 PMID:17472963 PMID:18451993

• The enzyme exhibits calcium-dependent phospholipase A2 and lysophospholipase activities, critical for membrane lipid remodeling. It also has O-acyltransferase activity, transferring fatty acyl chains to glycerol (PubMed:7794891).
  Related references:
  PMID:7794891

• PLA2G4A plays a vital role in embryo implantation and parturition by triggering prostanoid production, and is expressed in macrophages, neutrophils, fibroblasts, lung endothelium, and platelets (PubMed:25102815).
  Related references:
  PMID:25102815

• Subcellularly, it resides in the cytoplasm, Golgi apparatus membrane, and nucleus envelope, translocating to membranes upon calcium signaling.

• The protein is post-translationally regulated by phosphorylation at key serines (e.g., S505, S727) and ubiquitination, and contains isopeptide bonds. It belongs to the phospholipase A2 family and features a calcium-binding C2 domain.

• Disease-associated variants have been reported, linking PLA2G4A to lipid metabolism disorders and inflammatory conditions. Keywords include lipid degradation, leukotriene biosynthesis, and prostaglandin metabolism.

Experimental Guidance and Technical Tips

• This phospho-specific antibody targets human PLA2G4A at phosphorylated Ser505. Ensure sample preparation includes phosphatase inhibitors to preserve phosphorylation states.
• For Western blot, consider using stimulated (e.g., calcium ionophore, cytokine-treated) cell lysates where cPLA2α is activated and phosphorylated. The detected band should be approximately 85 kDa.
• For ELISA applications, pair with a total PLA2G4A capture antibody and use phospho-S505 peptide for competition assays to confirm specificity.
• Validate cross-reactivity in mouse or rat samples using species-specific controls, as the immunogen sequence may differ.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, including validated antibody pairs, optimized monoclonals/polyclonals, and bulk ancillary reagents. This anti-phospho-PLA2G4A antibody supports development of immunoassays targeting inflammatory and lipid signaling pathways, with CamelBio facilitating efficient sourcing and tailored supply chains. Our cross-species reactive, phosphorylation-specific antibodies accelerate biomarker and assay development from concept to clinical application.