Anti-Phospho-PKC zeta-T560 Rabbit Monoclonal Antibody for WB, ELISA - Q05513

Core Product Specifications and Parameters

Biological Background: PKC zeta Function and Localization

• Protein kinase C zeta (PKC zeta) is an atypical, calcium- and diacylglycerol-independent serine/threonine-protein kinase.
• It functions in the phosphatidylinositol 3-kinase (PI3K) pathway and MAP kinase cascades, playing roles in NF-kappa-B activation, mitogenic signaling, cell proliferation, and cell polarity.
• Required for insulin-dependent activation of AKT3 and contributes to glucose transporter SLC2A4/GLUT4 translocation in adipocytes.
• Participates in TNF-dependent NF-kappa-B activation by phosphorylating IKBKB, and is required for T-helper 2 (Th2) differentiation and allergic airway inflammation.
• Subcellular localization includes cytoplasm, endosomes, cell junctions, and membrane.
• Expressed predominantly in brain, with lower levels in lung, kidney, and testis.
• Involved in late synaptic long-term potentiation in hippocampal CA1 cells and long-term memory maintenance.
• Phosphorylates VAMP2 and LRRK1, regulating intracellular trafficking. Related references: PMID:17313651, PMID:36040231

Experimental Guidance and Technical Tips

• Consider validating phospho-specificity by treating samples with lambda phosphatase prior to WB analysis.
• Use gentle lysis buffers with phosphatase inhibitors to preserve the T560 phosphorylation signal.
• For WB, a band near 68 kDa is expected; use appropriate positive controls such as insulin- or EGF-stimulated cell lysates.
• The antibody’s cross-reactivity with rat suggests utility in rodent model studies; validate in the appropriate species context.
• ELISA protocols may benefit from a phospho-peptide blocking step to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, technical services, and consulting—from concept to clinic. For PKC zeta and other cell signaling targets, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Partner with us to streamline your kinase research and assay development.