Anti-Phospho-PKC (pan) (zeta)-T410 Rabbit pAb - P17252 / P05771 / Q05655 / Q02156 / P05129 / P24723 / P41743 / Q04759 / Q05513

Core Product Specifications and Parameters

Biological Background: PKC (pan) (zeta) Function and Localization

• PKC alpha (PRKCA) is a calcium-activated, phospholipid- and diacylglycerol-dependent serine/threonine kinase that regulates proliferation, apoptosis, differentiation, migration, and adhesion by phosphorylating key substrates including RAF1, BCL2, and CSPG4.
• It promotes cell proliferation via RAF1-mediated MAPK/ERK activation and protects glioma cells from apoptosis by inhibiting p53-dependent IGFBP3 expression and phosphorylating BCL2.
• Subcellular localization spans cytoplasm, membrane, and nucleus; during macrophage differentiation, PKC alpha translocates to the nucleus to support macrophage development, and it relocalizes to lamellipodia to modulate cell motility and desmosomal adhesion.
• In CD4(+) T cell chemotaxis, PKC alpha phosphorylates DOCK8, triggering dissociation from LRCH1 and activation of CDC42 to drive cell migration (PubMed:28028151). Related references: PMID:28028151
• Highly expressed in gliomas and breast cancers, PKC alpha acts as a tumor promoter and contributes to malignant phenotypes.
• In cardiomyocytes, phosphorylation of cardiac troponin T (TNNT2) reduces myofilament calcium sensitivity, and ERK1/2-dependent hypertrophic signaling leads to pathological growth and heart failure.
• During angiogenesis, PKC alpha supports endothelial migration and VEGFA-induced cell proliferation and tube formation; in platelets, it mediates CD36-dependent granule release and integrin activation for aggregation.
• Additional regulatory roles include phosphorylation of KLHL3 to modulate WNK4 interaction in angiotensin II signaling, and activation of LRRK1 to control RAB protein trafficking. Related references: PMID:25313067 PMID:36040231

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide surrounding Thr410 of human PKC zeta. Depending on sequence conservation, the antibody may detect multiple PKC family members when phosphorylated at the equivalent residue; validate specificity in your target isoforms.
• For WB, include phosphatase inhibitor cocktails during lysis and consider treating a control sample with lambda phosphatase to confirm phospho-specificity.
• For ELISA, test with phosphorylated and non-phosphorylated peptides to assess binding specificity.
• Optimize antibody dilution for each application and sample type; recommended to run appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research labs worldwide. This anti-phospho-PKC (pan) (zeta)-T410 antibody supports studies in cancer, cardiovascular, and immune signaling pathways. We offer reliable supply of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets—helping you bridge from concept to clinic.