Anti-Phospho-PKC (pan) (βII Ser660) Monoclonal Antibody for WB, IHC-P, ELISA - P17252 / P05771 / Q05655 / Q02156 / P24723 / Q04759

Core Product Specifications and Parameters

Biological Background: PKC (pan) Function and Localization

• Protein kinase C alpha (PRKCA) is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that regulates diverse cellular processes including proliferation, apoptosis, differentiation, migration, and adhesion.
• It plays a role in chemokine-induced T cell migration by phosphorylating DOCK8, leading to CDC42 activation. Related references: PMID:28028151
• PRKCA is involved in angiotensin II signaling by phosphorylating KLHL3, modulating WNK4 interactions and blood pressure regulation. Related references: PMID:25313067
• It phosphorylates LRRK1, which in turn phosphorylates RAB proteins involved in intracellular trafficking. Related references: PMID:36040231
• Subcellular localization includes cytoplasm, cell membrane, mitochondrion membrane, and nucleus, depending on cellular context and stimuli.
• The PKC family includes several isoforms; this antibody, generated against a phosphorylated peptide around Ser660 of PRKCA, recognizes phosphorylated forms of multiple PKC isoforms (alpha, beta, delta, epsilon, eta, theta) due to sequence conservation in the turn motif.
• PKC alpha contains C1 and C2 regulatory domains, including phorbol ester/DAG-binding zinc finger motifs, and is subject to multiple post-translational modifications including phosphorylation and acetylation.

Experimental Guidance and Technical Tips

• For Western blot (WB), validate the antibody in your specific sample system; consider induction with phorbol esters (e.g., PMA) to stimulate PKC phosphorylation.
• For immunohistochemistry (IHC) on paraffin sections (IHC-P), antigen retrieval conditions may need optimization; phospho-specific antibodies often require gentle retrieval to preserve phosphorylation.
• For ELISA, use the antibody for detecting phospho-PKC (pan) in cell lysates or purified proteins; include phosphatase inhibitors during sample preparation to maintain phosphorylation.
• Confirm specificity by treating samples with lambda phosphatase or using non-phosphorylated peptide competition.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined, one-stop access to IVD raw materials, technical services, and consulting for diagnostic manufacturers, laboratories, and research institutes. This phospho-PKC (pan) antibody supports investigations into key PKC signaling pathways relevant to oncology, cardiovascular biology, and inflammation. Beyond this product, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to accelerate your project from concept to clinic.