Anti-Phospho-PKC (pan) (gamma)-T514 Rabbit pAb for WB and ELISA - P17252 / P05771 / Q05655 / Q02156 / P05129 / P24723 / Q04759

Core Product Specifications and Parameters

Biological Background: PKC (pan) (gamma)-T514 Function and Localization

• Protein kinase C (PKC) family members are serine/threonine kinases activated by calcium, diacylglycerol (DAG), and phospholipids, regulating diverse cellular processes including proliferation, apoptosis, differentiation, and migration.
• PKC alpha (PRKCA), a widely expressed and representative isoform, is involved in both positive and negative regulation of the cell cycle and can promote cell growth via RAF1/ERK signaling or induce growth arrest through CDK inhibitors.
• Exhibits anti-apoptotic functions by phosphorylating BCL2 and suppressing p53/TP53-mediated activation of IGFBP3, and is highly expressed in many cancers where it can act as a tumor promoter.
• Undergoes stimulus-dependent translocation between cytoplasm, cell membrane, mitochondrial membrane, and nucleus, enabling context-specific signaling.
• In cardiac function, PKC alpha regulates cardiomyocyte hypertrophy, myocardial contractility, and heart failure progression; it phosphorylates cardiac troponin T (TNNT2) to modulate calcium sensitivity.
• In angiogenesis, is required for endothelial cell migration, adhesion to vitronectin, and VEGFA-induced signaling, stabilizing VEGFA mRNA post-transcriptionally.
• This antibody specifically recognizes PKC proteins phosphorylated at the conserved threonine 514 residue (T514 in PKC alpha; equivalent activation loop sites in beta, delta, epsilon, gamma, eta, and theta isoforms), which is critical for full kinase activity.
• Phosphorylates KLHL3 at Ser-52 in response to angiotensin II signaling, decreasing the interaction between KLHL3 and WNK4. Related references: PMID:25313067
• Phosphorylates DOCK8, leading to its dissociation from LRCH1 and subsequent activation of CDC42 GTPase during chemokine-induced T cell migration. Related references: PMID:28028151
• Phosphorylates LRRK1, which in turn phosphorylates RAB proteins involved in intracellular trafficking. Related references: PMID:36040231

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around T514 of human PKC alpha; this design targets the phosphorylation-specific epitope across multiple PKC isoforms.
• For Western blot, include phosphatase inhibitors in lysis and wash buffers to preserve phosphorylation, and consider using BSA-based blocking solutions to reduce non-specific binding.
• ELISA detection may be performed on cell or tissue lysates; optimize coating and detection conditions for phospho-proteins, and validate specificity using non-phosphorylated peptide controls.
• Because the antibody recognizes several PKC isoforms, isoform-specific responses should be verified using relevant knockout cell lines or isoform-selective inhibitors.
• Predicted bands may range from 59–83 kDa depending on the isoform; confirm molecular weight in the sample system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this anti-Phospho-PKC (pan) (gamma)-T514 rabbit polyclonal antibody as part of our extensive IVD raw-material portfolio for kinase signaling and disease research. We specialize in supplying validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents—streamlining your development from concept to clinic. Our one-stop platform facilitates access to rare-target raw materials and technical consulting, supporting diagnostic manufacturers and research labs worldwide.