Anti-Phospho-PKC mu (S744/748) Rabbit Polyclonal Antibody for WB and ELISA - Q15139

Core Product Specifications and Parameters

Biological Background: PRKD1/PKC mu Function and Localization

• Serine/threonine-protein kinase D1 (PRKD1/PKC mu) converts transient diacylglycerol (DAG) signals into prolonged physiological effects downstream of PKC, regulating MAPK8/JNK1 and Ras signaling, Golgi integrity, cell survival, migration, differentiation, and proliferation. Related references: PMID:10764790 PMID:12505989 PMID:12637538
• It phosphorylates EGFR on dual threonine residues, suppressing EGF-induced JNK activation, and phosphorylates RIN1 to modulate Ras signaling. Related references: PMID:10523301
• Under oxidative stress, phosphorylation at Tyr-463 via SRC-ABL1 activates the IKK complex and NF-kappa-B, promoting cell survival. Related references: PMID:12505989
• In cardiac hypertrophy, PRKD1 phosphorylates HDAC5, triggering nuclear export and MEF2A activation; it also mediates TNNI3 phosphorylation, reducing myofilament calcium sensitivity. Related references: PMID:18332134
• The PRKD1-HDAC5 pathway is essential for VEGFA-induced angiogenesis; PRKD1 is also required for HDAC7 nuclear export during endothelial proliferation and migration. Related references: PMID:19211839
• Upon genotoxic stress, caspase-3 cleaves PRKD1 at Asp-378, activating its kinase function and sensitizing cells to apoptosis. Related references: PMID:10764790
• PRKD1 directly phosphorylates TRPV1, modulates TLR5 signaling in inflammatory responses, and activates NLRP3 inflammasome assembly. Related references: PMID:15471852 PMID:17442957
• Subcellularly, PRKD1 localizes to the cytoplasm, cell membrane, and the trans-Golgi network, where it is critical for vesicle fission and secretory trafficking.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide spanning Ser744 and Ser748 of mouse PKC mu; antibody specificity should be verified using phosphatase-treated or dephosphorylated samples.
• For Western blotting, a band around 102 kDa is expected; consider using appropriate molecular weight markers and validate the signal in relevant human, mouse, or rat cell lysates.
• ELISA application can be used to detect phospho-PKC mu levels; optimize coating conditions and detection antibodies for the specific assay format.
• Given cross-reactivity with multiple species, ensure the antibody is validated in the target species’ tissue or cell line of interest.
• Phosphorylation at S744/748 is dynamic; include appropriate stimulation (e.g., phorbol ester, growth factors) and inhibition controls to confirm signal specificity.

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CamelBio connects diagnostic manufacturers, research laboratories, and biotech institutes with a comprehensive selection of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. If you require bulk quantities of this phosphorylation-specific PKC mu antibody or need support in sourcing related reagents for signal transduction studies, our team can streamline procurement and supply. Explore our offerings to bridge your research from concept to clinic with reliable, one-stop access.