Anti-Phospho-PKC delta (T505) Rabbit Polyclonal Antibody for WB, ELISA - Q05655

Core Product Specifications and Parameters

Biological Background: PKC delta Function and Localization

• Protein kinase C delta type (PKC delta), encoded by the PRKCD gene, is a calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and survival.
• Functions as a pro-apoptotic protein during DNA damage-induced apoptosis by activating the BCLAF1-mediated p53/TP53 gene transcription and forming a complex with ABL1 to trigger p53-independent apoptosis. Related references: PMID:21406692, PMID:21810427
• Mediates cell cycle regulation at G1/S and G2/M checkpoints, upregulating CDKN1A/p21 and phosphorylating CDK1, and also promotes cancer cell survival by activating AKT1, NF-kappa-B, and ERK1/2 pathways. Related references: PMID:15774464
• Regulates immune cell functions, including phosphorylation and activation of CARD9 in antifungal immunity, and is required for NADPH oxidase activation in neutrophils via NCF1 phosphorylation. Related references: PMID:19801500, PMID:11748588
• Phosphorylates multiple substrates such as MCL1 (targeting it for degradation), PLSCR3 (increasing cardiolipin exposure on the mitochondrial outer membrane), and 14-3-3 proteins. Related references: PMID:12649167
• Localizes to multiple subcellular compartments including cytoplasm, perinuclear region, nucleus, cell membrane, mitochondrion, and endomembrane system, reflecting its diverse signaling roles.
• Classified under keywords: Apoptosis, tumor suppressor, zinc-finger, kinase, phosphoprotein, indicating its regulatory functions.

Experimental Guidance and Technical Tips

• This antibody is raised against a synthetic phosphorylated peptide around T505 of human PKC delta; therefore, it is expected to specifically recognize the phosphorylated T505 form. Consider using phosphatase-treated lysates to confirm phosphorylation-dependent detection.
• Predicted molecular weight of PKC delta is 78 kDa. In Western blot, a band around this size may be observed, but migration could be influenced by post-translational modifications. Validate in the appropriate sample system.
• For ELISA, use the phosphorylated peptide as a positive control and assess cross-reactivity with the non-phosphorylated counterpart to ensure specificity.
• Since PKC delta is activated and phosphorylated upon various stimuli, stimulation with phorbol esters (e.g., PMA) or DNA-damaging agents may enhance the signal in cellular models.
• As a polyclonal antibody, lot-to-lot variability is possible; it is advisable to include appropriate positive and negative controls in each experiment.

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