Anti-Phospho-PKC delta-S645 Rabbit Polyclonal Antibody for WB, ELISA - Q05655

Core Product Specifications and Parameters

Biological Background: Protein kinase C delta type Function and Localization

• Protein kinase C delta type (PKCδ, nPKC-delta) is encoded by the PRKCD gene and belongs to the novel PKC subfamily, functioning as a calcium-independent, phospholipid- and diacylglycerol-dependent serine/threonine-protein kinase.
• Exhibits dual roles in apoptosis: pro-apoptotic during DNA damage-induced cell death by activating p53/TP53 and BCLAF1, and anti-apoptotic in cytokine receptor-mediated survival signaling. Related references: PMID:21406692 PMID:21810427
• Plays a critical role in innate immunity by regulating NADPH oxidase activity and superoxide production in neutrophils and eosinophils upon stimulation with fMLP, IL5, or PMA. Related references: PMID:19801500 PMID:11748588
• In platelets, PKCδ acts as a negative regulator of filopodia formation and actin polymerization by interacting with VASP, and differentially modulates dense granule secretion downstream of PAR and CD36 receptors. Related references: PMID:16940418 PMID:19587372
• Phosphorylates a wide range of substrates including MUC1, ELAVL1, PLSCR3, MCL1, and SMPD1, thereby influencing processes such as cardiolipin externalization, apoptosis, and sphingomyelinase secretion. Related references: PMID:11877440 PMID:18285462 PMID:12649167 PMID:17303575
• Localizes to multiple cellular compartments including cytoplasm, perinuclear region, nucleus, cell membrane, and mitochondrion, enabling context-specific signaling.
• Undergoes extensive post-translational modifications, including phosphorylation at several serine and threonine residues (e.g., S645) that regulate its activity, localization, and protein interactions.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphopeptide corresponding to S645 of human PKCδ; consider using non-phosphorylated peptide blocking or phosphatase treatment to confirm phospho-specificity.
• For western blotting, the expected molecular weight is ~78 kDa; include appropriate loading controls and validate with your sample system.
• The product shows cross-reactivity with mouse and rat PKCδ based on sequence conservation; perform further validation for non-human models.
• ELISA applications may benefit from pairing with a capture antibody or optimizing coating conditions; empirical titration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers comprehensive one-stop access to IVD raw materials, technical services, and consulting—from early research to clinical application. This phospho-PKCδ-S645 polyclonal antibody supports studies in apoptosis, cell signaling, and cancer biology. We provide validated antibody pairs, optimized polyclonal/monoclonal antibodies, and bulk ancillary reagents to streamline your assay development and sourcing needs.