Anti-Phospho-PI3 Kinase p55-gamma (PIK3R3) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q92569

Core Product Specifications and Parameters

Biological Background: PI3 Kinase Regulatory Subunit Gamma (PIK3R3) Function and Localization

• Encoded by the PIK3R3 gene, PI3 kinase regulatory subunit gamma (also known as p55PIK or p55-gamma) is a 55 kDa regulatory subunit of class IA phosphatidylinositol 3-kinases, characterized by its SH2 domain.
• Binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain, modulating their kinase activity; during insulin stimulation, it also associates with IRS-1 to propagate downstream signals.
• Belongs to the PI3K regulatory subunit family that includes p85 and p55 isoforms, contributing to the diversity of PI3K signaling complexes.
• Exhibits highest expression in brain and testis, with lower levels in adipose tissue, kidney, heart, lung, and skeletal muscle, suggesting tissue-specific regulatory roles.
• Classified as a phosphoprotein subject to alternative splicing, and has been identified in high-throughput proteomics studies, highlighting its post-translational regulation and inclusion in reference datasets.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to Y199, so the antibody specifically recognizes the phosphorylated form; include phosphatase inhibitors during sample preparation to preserve the phosphorylation signal.
• For Western blotting, validate the detected band at ~55 kDa using insulin-stimulated or pervanadate-treated cell lysates to confirm phospho-specificity.
• In IF/ICC, optimize permeabilization and fixation conditions (e.g., 4% PFA followed by methanol) to retain the phosphorylation epitope; co-stain with a total PI3K p55 antibody to assess activation dynamics.
• Since cross-reactivity with mouse is reported, the antibody can be considered for mouse models; however, verify performance in the relevant tissue or cell type first.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials and technical support. This Phospho-PI3Kinase p55-gamma antibody targets a key insulin signaling protein, and CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for assay development. For programs requiring specialized or rare target raw materials, CamelBio offers customized sourcing solutions to streamline your research-to-clinic pipeline.