Anti-Phospho-PERK (T982) Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q9NZJ5

Core Product Specifications and Parameters

Biological Background: PERK Function and Localization

• EIF2AK3/PERK is a metabolic-stress sensing kinase that phosphorylates eIF2-alpha (EIF2S1) on Ser51 in response to endoplasmic reticulum (ER) stress, a central step in the unfolded protein response (UPR).
  Related references: PMID:10026192, PMID:10677345, PMID:11907036
• Phosphorylation of eIF2-alpha by PERK inhibits global cap-dependent translation while selectively enhancing translation of stress-responsive mRNAs such as ATF4 and QRICH1, enabling cellular reprogramming under stress.
  Related references: PMID:10026192, PMID:10677345, PMID:31023583
• PERK-mediated UPR enhances mitochondrial oxidative phosphorylation by promoting ATF4-dependent expression of COX7A2L/SCAF1, which increases respiratory chain supercomplex formation.
  Related references: PMID:31023583
• PERK is inhibited at mitochondria-ER contact sites by ATAD3A, protecting mitochondria from the global UPR translational shutdown.
  Related references: PMID:39116259
• Beyond eIF2-alpha, PERK also phosphorylates NFE2L2/NRF2, triggering its release from KEAP1 and subsequent nuclear accumulation to activate antioxidant gene expression.
• PERK is an ER-resident transmembrane protein and is ubiquitously expressed, with highest levels in secretory tissues.
• The protein has a molecular weight of ~125 kDa and belongs to the Ser/Thr protein kinase family, with additional tyrosine kinase activity.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide corresponding to the phosphorylation site Thr982 of human PERK; it specifically recognizes phosphorylated PERK and will not detect the unphosphorylated form. Verify phospho-specificity in your experimental system, e.g., by phosphatase treatment.
• For western blot (WB), a band of approximately 125 kDa is expected. Optimize blocking and antibody dilution to minimize non-specific binding, and consider loading appropriate controls (e.g., stress-induced vs. unstressed cell lysates).
• For immunohistochemistry (IHC-P), antigen retrieval conditions may influence staining; validate with known positive and negative tissue samples and consider using a phospho-specific detection protocol.
• The antibody cross-reacts with mouse and rat PERK, enabling translational studies; however, confirm reactivity in your specific tissue or cell lines, as phosphorylation status may vary.
• For ELISA applications, this antibody can be used as a detection or capture reagent, but assay conditions (coating, blocking, detection method) must be optimized for phospho-specific detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and academic institutes with a comprehensive, one-stop solution for IVD raw material sourcing. This anti-phospho-PERK (T982) antibody supports research into ER stress, unfolded protein response, and related diseases. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom rare-target sourcing to streamline your assay development from concept to clinic.