Anti-Phospho-PDGFRB (Y1021) Rabbit Polyclonal Antibody for WB, ELISA - P09619

Core Product Specifications and Parameters

Biological Background: PDGFRB Function and Localization

• Platelet-derived growth factor receptor beta (PDGFRB, also designated CD140b) is a cell-surface tyrosine kinase receptor that binds homodimers of PDGFB and PDGFD, as well as PDGFA/PDGFB heterodimers, initiating intracellular signaling.
• It plays a critical role in embryonic development, particularly in the cardiovascular system, by promoting the proliferation, migration, and recruitment of pericytes and vascular smooth muscle cells to nascent blood vessels.
• Ligand-induced dimerization triggers autophosphorylation of specific tyrosine residues (including Y1021), which serve as docking sites for SH2-domain-containing signaling proteins, activating pathways such as PLCG1–Ca²⁺/PKC, PI3K–AKT, and RAS–MAPK cascades.
• PDGFRB is essential for normal kidney glomerular development, where it mediates mesangial cell recruitment and capillary branching, and it drives neointima formation following vascular injury.
• The receptor localizes primarily to the cell membrane but undergoes rapid internalization into cytoplasmic vesicles and lysosomes upon activation, a mechanism that attenuates downstream signaling.
• As a proto-oncogene, PDGFRB is implicated in oncogenesis through chromosomal rearrangements (e.g., in chronic myelomonocytic leukemia) and overexpression in glioblastoma and sarcomas, highlighting its role in cell proliferation, chemotaxis, and survival.
• Alternative splicing generates multiple isoforms, and the protein is extensively modified by N-linked glycosylation and disulfide bonds within its immunoglobulin-like domains.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide corresponding to residues around phosphorylated Y1021; to confirm phosphorylation-specific binding, consider performing phosphatase treatment of cell lysates prior to Western blot or using site-directed mutagenesis of the target tyrosine.
• For Western blot, the predicted molecular weight of PDGFRB is ~124 kDa; however, the mature glycoprotein often migrates at a higher apparent mass (150–180 kDa) due to glycosylation. Use appropriate molecular weight markers and validate band identity with positive (e.g., PDGF-stimulated) and negative (serum-starved) controls.
• The antibody is suitable for ELISA; when designing capture/detection formats, pair it with a total PDGFRB antibody or a different phospho‑specific clone to enhance assay specificity.
• Cross-reactivity is expected with human, mouse, and rat PDGFRB; verify performance in the species and tissue of interest using the recommended starting dilutions for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with reliable, end‑to‑end access to IVD raw materials—from concept to clinic. This phospho‑specific PDGFRB antibody supports assay development in vascular biology, oncology, and signaling research. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing services for rare targets, helping you accelerate biomarker discovery and diagnostic kit production.