Anti-Phospho-PDGFRB (Tyr751) Rabbit Polyclonal Antibody for WB, ELISA - P09619

Core Product Specifications and Parameters

Biological Background: PDGFRB Function and Localization

• Platelet-derived growth factor receptor beta (PDGFRB) is a transmembrane tyrosine kinase that serves as a receptor for PDGFB, PDGFD, and PDGFA-PDGFB heterodimers, playing crucial roles in cell proliferation, survival, migration, and differentiation.
• PDGFRB signaling is essential for embryonic development, particularly in blood vessel formation, where it promotes proliferation and recruitment of pericytes and smooth muscle cells to endothelial cells, and is required for normal cardiovascular system development.
• This receptor is a key member of the PDGFR family, which includes PDGFRA, and can form heterodimers with PDGFRA upon ligand stimulation, modulating downstream signaling cascades.
• Subcellularly, PDGFRB primarily localizes to the cell membrane but can also be found in cytoplasmic vesicles and the lysosome lumen, consistent with its activation-dependent receptor trafficking and down-regulation.
• Phosphorylation of tyrosine 751 (Tyr751) is a critical event in receptor activation, creating a docking site for downstream signaling molecules such as PI3K, contributing to AKT pathway activation and promoting cell survival and migration.
• The receptor is classified as a developmental protein and proto-oncogene, with involvement in vascular smooth muscle migration and neointima formation at injury sites, and is required for normal pericyte recruitment in kidney glomeruli.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to residues surrounding Tyr751 of human PDGFRB; use a non-phosphorylated peptide control to confirm phospho-specificity in your assay.
• For Western blotting, consider treating cells with PDGFB to induce robust Tyr751 phosphorylation, and use phosphatase inhibitors during lysis to preserve the phospho-signal.
• In ELISA applications, coating with the phospho-peptide and detection with an anti-phospho-tyrosine antibody can help validate target engagement.
• Cross-reactivity with mouse PDGFRB suggests utility in murine models, but verify performance in the relevant tissue or cell line due to potential species-specific variations.
• The predicted molecular weight is approximately 124 kDa; a band shift upon stimulation may be observed, reflecting glycosylation or other modifications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive range of IVD raw materials, including this anti-phospho-PDGFRB (Tyr751) antibody, to support research and diagnostic development in cardiovascular biology and oncology. As your integrated sourcing partner, we can provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored assistance for rare target raw-material procurement. Let us help streamline your project from concept to clinic.