Anti-Phospho-PDGFR alpha-Y754 Rabbit Polyclonal Antibody for WB, ELISA - P16234

Core Product Specifications and Parameters

Biological Background: PDGFR alpha Function and Localization

• Platelet-derived growth factor receptor alpha (PDGFRA, also designated PDGFR2, CD140A) is a member of the type III receptor tyrosine kinase family, characterized by five immunoglobulin-like extracellular domains and a split intracellular tyrosine kinase domain.
• It functions as a cell-surface receptor for platelet-derived growth factors (PDGFA, PDGFB, PDGFC), playing a central role in embryonic development by regulating cell proliferation, survival, migration, and chemotaxis.
• PDGFRA signaling is essential for normal skeleton formation, cephalic closure, and the development of gastrointestinal mucosa and intestinal villi, as well as in recruiting mesenchymal cells during organogenesis.
• Ligand binding triggers receptor dimerization and autophosphorylation at multiple tyrosine residues (including Y754), leading to the activation of downstream pathways such as PI3K-AKT, MAPK/ERK, and STAT signaling cascades.
• The activation of PLCG1 by PDGFRA results in the production of diacylglycerol and inositol 1,4,5-trisphosphate, leading to the mobilization of cytosolic Ca2+ and activation of protein kinase C.
• Subcellularly, PDGFRA is predominantly found at the cell membrane, but is also detected in the Golgi apparatus and primary cilia, reflecting its dynamic localization and trafficking.
• In addition to its developmental roles, PDGFRA participates in platelet activation, agonist secretion, and thrombin-induced platelet aggregation, highlighting its importance in hemostasis and wound healing.
• PDGFRA is widely expressed in tissues, including brain, fibroblasts, smooth muscle, heart, and embryos, and is upregulated in various cancers, making it a target of interest in oncology research.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y754; verify phosphorylation specificity by comparing signals before and after phosphatase treatment or by using a non-phosphorylated peptide as a blocking control.
• For Western blot, the expected molecular weight is ~123 kDa, but glycosylation and other modifications may cause slight shifts. Validate the antibody under reducing and non-reducing conditions in the relevant cell lysates.
• In ELISA, optimize coating conditions and detection steps to quantify phosphorylated PDGFRA in cell or tissue extracts. Include positive and negative controls to ensure assay specificity.
• Cross-reactivity is listed as human; if working with other species, check sequence homology around Y754 to predict potential cross-reactivity and perform experimental validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering high-quality antibodies like this anti-phospho-PDGFR alpha-Y754 rabbit pAb to support your diagnostic and research workflows. We provide validated antibody pairs, bulk reagents, and customized procurement solutions for rare or specialized targets, streamlining your journey from concept to clinic. Let us help you accelerate PDGFRA-related studies with reliable, ready-to-use materials.