Anti-Phospho-Paxillin (Y118) Rabbit Monoclonal Antibody for WB, ELISA - P49023

Core Product Specifications and Parameters

Biological Background: Paxillin Function and Localization

• Paxillin is a cytoskeletal protein that mediates actin-membrane attachment at focal adhesions, anchoring the actin cytoskeleton to the extracellular matrix.
• It functions as a molecular scaffold, recruiting signaling and structural proteins such as TRIM15 to sites of cell adhesion.
• Localizes to the cytoplasm, cytoskeleton, and specifically to cell junctions and focal adhesions, with enrichment at the cell cortex.
• Contains LIM domains and zinc-binding motifs, characteristic of proteins involved in cell adhesion and cytoskeletal organization.
• Classified as a phosphoprotein, with phosphorylation at tyrosine 118 (Y118) playing a key regulatory role in focal adhesion dynamics and cell migration.
• Undergoes alternative splicing, producing multiple isoforms.

Experimental Guidance and Technical Tips

• For Western blot, add phosphatase inhibitors during lysis to preserve Y118 phosphorylation. A band near 65 kDa is expected.
• Confirm phospho-specificity by treating lysates with lambda phosphatase or by competitive blocking with the non-phosphorylated peptide.
• In ELISA, pair this antibody with a total Paxillin detection antibody to normalize phospho-signal.
• Validate reactivity in human, mouse, and rat cell lines known for active focal adhesion signaling (e.g., fibroblasts, cancer lines).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-phospho-Paxillin (Y118) monoclonal antibody supports focal adhesion and cell adhesion research, offering a reliable tool for pathway analysis. Through our integrated supply chain, we deliver validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary materials, and custom sourcing for rare targets to accelerate your assay development and clinical translation.