Anti-Phospho-PAK2 (S20) Rabbit Monoclonal Antibody for WB, ELISA - Q13177

Core Product Specifications and Parameters

Biological Background: PAK2 Function and Localization

• Serine/threonine-protein kinase PAK2, also known as PAK65, Gamma-PAK, or p58, is a member of the p21-activated kinase (PAK) family.
• Acts as a downstream effector of the small GTPases CDC42 and RAC1; binding leads to autophosphorylation and kinase activation. Related references: PMID:7744004
• Phosphorylates diverse substrates including histone H4 to promote nucleosome assembly, JUN for EGF-induced proliferation, and MAPK4/6 to regulate F-actin polymerization. Related references: PMID:21724829, PMID:21177766, PMID:21317288
• Caspase-mediated cleavage generates PAK-2p34, which translocates to the nucleus and promotes apoptosis via JNK signaling; also phosphorylates MKNK1 to reduce cellular translation. Related references: PMID:12853446, PMID:16617111, PMID:9171063, PMID:15234964
• Localizes to the cytoplasm, nucleus, perinuclear region, and membrane, and exerts kinase-independent functions in mitotic spindle orientation when associated with ARHGEF7 and GIT1. Related references: PMID:19273597, PMID:19923322
• Ubiquitously expressed with higher levels in skeletal muscle, ovary, thymus, and spleen; post-translationally modified by acetylation, myristoylation, and ubiquitin conjugation.
• Also phosphorylates and inhibits CASP7, preventing apoptosis. Related references: PMID:21555521, PMID:27889207

Experimental Guidance and Technical Tips

• Since the immunogen is a synthetic phosphorylated peptide around serine 20, this antibody is expected to specifically recognize PAK2 phosphorylated at S20; consider using phosphatase inhibitors in sample preparation to preserve phosphorylation.
• For Western blot, validation in human cell lines expressing PAK2 and confirm band at ~58 kDa; cross-reactivity with mouse and rat allows studies in these species, but sequence homology around S20 should be checked.
• In ELISA, use a non-phosphorylated peptide control to verify phospho-specificity.
• Include appropriate controls (e.g., untreated vs. phosphatase-treated lysates) to confirm phospho-dependence.
• Optimal working dilutions must be determined empirically; start with manufacturer's recommendations if available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a one-stop sourcing channel for high-quality IVD raw materials and technical support. This Phospho-PAK2-S20 Rabbit mAb supports studies on PAK2-mediated signaling pathways, and we offer a comprehensive range of matching antibody pairs, detection reagents, and custom procurement for rare targets. Contact us to streamline your IVD development pipeline from concept to clinic.