Anti-Phospho-p90Rsk/RSK1/RPS6KA1 (S380) Rabbit Monoclonal Antibody for WB, ELISA - Q15418

Core Product Specifications and Parameters

Biological Background: RPS6KA1 (RSK1) Function and Localization

• RPS6KA1 (RSK1) is a serine/threonine kinase of the p90 ribosomal S6 kinase family, also known as p90RSK1, MAPKAPK1, and RSK1, encoded by the RPS6KA1 gene; alternative splicing generates multiple isoforms.
• Acts downstream of ERK1/2 (MAPK3/MAPK1) signaling to mediate mitogenic and stress-induced activation of transcription factors CREB1, ETV1/ER81, and NR4A1/NUR77, thereby coupling MAPK cascade to transcriptional regulation. Related references: PMID:10679322 | PMID:12213813 | PMID:15117958
• Regulates cap-dependent translation by phosphorylating ribosomal protein S6 (RPS6) via an mTOR-independent mechanism and eukaryotic translation initiation factor 4B (EIF4B), enhancing its affinity for the EIF3 complex. Related references: PMID:17360704 | PMID:16763566
• Modulates mTOR signaling by directly phosphorylating TSC2 at Ser1798 (inhibiting its GAP activity) and phosphorylating RPTOR and DEPTOR, thus influencing mTORC1 and mTORC2 activities. Related references: PMID:15342917 | PMID:22017876
• Promotes cell survival by phosphorylating and inactivating the pro-apoptotic factors BAD and DAPK1, and supports hepatic stellate cell survival through CEBPB phosphorylation. Related references: PMID:10679322 | PMID:16213824 | PMID:11684016
• Regulates cell cycle progression by phosphorylating CDKN1B (p27Kip1), promoting its 14-3-3 binding and cytoplasmic retention, and controls cell migration via EPHA2 phosphorylation at Ser897. Related references: PMID:18508509 | PMID:18813292 | PMID:26158630
• Localizes to both the nucleus and cytoplasm, enabling dual functions in transcriptional regulation and cytoplasmic signaling.
• Contains two non-identical kinase domains, requires ATP and magnesium for activity, and is regulated by multiple phosphorylation events, including Ser380 in the linker region which is critical for activation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide around Ser380 of human RSK1; consider using lambda phosphatase treatment or Ser380 non-phosphorylatable mutants in WB to confirm phospho-specificity.
• For WB, the predicted molecular weight is ~83 kDa; use appropriate molecular weight markers and validate with known positive controls (e.g., EGF-stimulated cells) due to RSK1 phosphorylation-dependent mobility shifts.
• In ELISA, optimize coating conditions (pH, peptide concentration) to maintain phospho-epitope integrity, and include non-phospho peptide controls to ensure specificity.
• Cross-reacts with mouse and rat RSK1; verify sequence homology around the phospho-site for these species to anticipate detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target procurement to support diagnostic manufacturers from concept to clinic. This phospho-RSK1 Rabbit mAb targets a central kinase in MAPK-driven cancer and stress pathways and complements our portfolio of signal transduction antibodies for assay development. Let us bridge your research and production needs with tailored sourcing solutions and technical consultations.