Anti-Phospho-p70 S6 Kinase 1-S371 Rabbit Polyclonal Antibody for WB and ELISA - P23443

Core Product Specifications and Parameters

Biological Background: p70 S6 Kinase 1 Function and Localization

• RPS6KB1 (p70 S6 kinase 1) is a serine/threonine kinase that acts downstream of mTORC1 signaling in response to growth factors and nutrients, promoting cell proliferation, growth, and cell cycle progression. Related references: PMID:11500364 PMID:12801526 PMID:14673156
• It regulates cap-dependent translation by phosphorylating EIF4B, RPS6, and by inhibiting EEF2K, thereby activating EEF2, as well as promoting pioneer round translation via POLDIP3/SKAR. Related references: PMID:11500364 PMID:12801526 PMID:14673156 PMID:15341740
• Under nutrient deprivation, inactive RPS6KB1 binds the EIF3 complex; upon mitogenic stimulation, mTORC1 phosphorylation causes dissociation and activation. Related references: PMID:16286006
• The kinase mediates cell survival by phosphorylating the pro-apoptotic factor BAD (by similarity), and by phosphorylating mitochondrial URI1 to release PP1CC, which dephosphorylates RPS6KB1 at Thr-412 in a negative feedback loop. Related references: PMID:17936702
• RPS6KB1 participates in feedback inhibition of mTORC2/AKT signaling by phosphorylating SIN1, MTOR, RICTOR, and DEPTOR. Related references: PMID:15899889 PMID:19720745 PMID:19935711 PMID:22017876
• It contributes to TNF-induced insulin resistance by phosphorylating IRS1 on serine residues, leading to IRS1 degradation. Related references: PMID:18952604
• The kinase phosphorylates and activates CAD, a key enzyme in pyrimidine biosynthesis, and EPRS to promote fatty acid uptake in adipocytes. Related references: PMID:23429703 PMID:28178239
• Subcellularly, RPS6KB1 localizes to the cytoplasm, nucleus, mitochondria outer membrane, and synapse, reflecting its diverse signaling roles.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to Ser371 of human RPS6KB1; we recommend validating the antibody’s specificity for the phosphorylated form using phosphatase-treated lysates or competing non-phosphorylated peptide.
• For Western blot, the predicted molecular weight is approximately 59 kDa (though it may migrate around 70 kDa due to phosphorylation). Include protease and phosphatase inhibitors during sample preparation.
• For ELISA, consider using the phosphorylated peptide as a positive control and the non-phosphorylated peptide to assess phospho-specificity.
• Validate cross-reactivity in mouse samples independently; human and mouse sequences are highly conserved around Ser371.
• Optimization of antibody dilution and blocking conditions may be necessary for different experimental setups.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop IVD raw-material sourcing partner, connecting diagnostic developers and researchers with validated reagents such as this anti-phospho-p70 S6 Kinase 1 antibody for mTOR pathway studies. Beyond phospho-specific antibodies, we supply optimized monoclonal/polyclonal antibodies, validated antibody pairs, bulk ancillary reagents, and can source rare-target raw materials to support your assay development from concept to clinic.