Anti-Phospho-p53-S15 Polyclonal Antibody for WB, IF/ICC, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 Function and Localization

• Tumor suppressor protein that induces cell cycle arrest, DNA repair, or apoptosis depending on cellular context and physiological signals.
  Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Pro-apoptotic activity is mediated through stimulation of BAX and FAS, or repression of Bcl-2; interactions with ASPP proteins modulate this activity.
  Related references: PMID:12524540, PMID:17189187
• Regulates circadian rhythms by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2.
  Related references: PMID:24051492
• Localizes to multiple subcellular compartments including the nucleus, cytoplasm, mitochondria, endoplasmic reticulum, and centrosome.
• Undergoes extensive post-translational modifications such as phosphorylation, acetylation, methylation, and ubiquitination, which regulate its stability and activity.
• Expressed ubiquitously, with multiple isoforms showing tissue-dependent expression patterns in normal tissues.
• Isoform 4 suppresses transactivation activity, while isoform 7 inhibits isoform 1-mediated apoptosis, highlighting functional diversity among splice variants.

Experimental Guidance and Technical Tips

• This antibody targets phosphorylated Serine 15 of human p53, a key phosphorylation site involved in DNA damage response. Ensure that samples are properly lysed and treated with phosphatase inhibitors to preserve phosphorylation.
• For Western blot, a band around 44 kDa is expected. Use appropriate loading controls and validate in the relevant cell lines or tissue lysates.
• For immunofluorescence, fixation and permeabilization conditions should be optimized; consider using a phospho-specific blocking buffer to reduce background.
• In ELISA, the antibody may be used to capture or detect phosphorylated p53; titrate to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research labs, and institutes streamlined access to high-quality IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. This phospho-p53-S15 antibody supports cancer research and DNA damage studies, and CamelBio ensures reliable supply and technical consultation for your assay development needs.