Anti-Phospho-p38 MAPK (Y182) Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q16539

Core Product Specifications and Parameters

Biological Background: Mitogen-activated Protein Kinase 14 (MAPK14/p38α) Function and Localization

• MAPK14, also known as p38α, is a serine/threonine kinase central to the MAP kinase signal transduction pathway, activated by pro-inflammatory cytokines, physical stress, and pathogens. It phosphorylates numerous transcription factors (ATF1, ATF2, ATF6, ELK1, TP53, MEF2C, MEF2A) and downstream kinases (MAPKAPK2/MK2, MAPKAPK3/MK3, RPS6KA5/MSK1, RPS6KA4/MSK2) to orchestrate cellular responses (PubMed:9687510, PubMed:9792677, PubMed:10330143, PubMed:9430721, PubMed:9858528). Related references: PMID:9687510 PMID:9792677 PMID:10330143 PMID:9430721 PMID:9858528

• Activation of downstream kinases by MAPK14 leads to chromatin remodeling and immediate-early gene induction (via MSK1/MSK2-mediated histone H3 phosphorylation) as well as post-transcriptional regulation through MK2/MK3-dependent phosphorylation of ZFP36 and ELAVL1 (PubMed:9687510, PubMed:9792677, PubMed:11154262). Related references: PMID:9687510 PMID:9792677 PMID:11154262

• MAPK14 also controls protein turnover (via SIAH2 ubiquitin ligase, PubMed:17003045), autophagy (through ATG9, PubMed:19893488), clathrin-mediated endocytosis (by modulating RAB5A effectors, PubMed:16932740), and ectodomain shedding (via ADAM17 phosphorylation, PubMed:20188673). Related references: PMID:17003045 PMID:19893488 PMID:16932740 PMID:20188673

• This kinase is essential for mid-fetal placental vascular development and stress-induced erythropoiesis via EPO regulation (PubMed:10943842), and it promotes NLRP1 inflammasome-associated pyroptosis upon ribosome collisions (PubMed:35857590). Related references: PMID:10943842 PMID:35857590

• Isoforms MXI2 and EXIP exhibit functional diversity: MXI2 is activated by mitogens and oxidative stress but poorly phosphorylates ELK1 and ATF2, while EXIP may facilitate early apoptosis.

• Subcellular localization is predominantly cytoplasmic and nuclear. MAPK14 is highly expressed in brain, heart, placenta, pancreas, and skeletal muscle, with lower levels in lung, liver, and kidney.

• Phosphorylation at Tyr-182 (Y182) is a critical activation marker; the antibody specifically recognizes this phospho-epitope, making it a valuable tool for studying stress and inflammatory signaling cascades.

Experimental Guidance and Technical Tips

• This phospho-specific rabbit monoclonal antibody was generated using a synthetic phosphorylated peptide corresponding to the region around Tyr-182 of human MAPK14. It is expected to selectively detect the phosphorylated (active) form of p38 MAPK in WB, IHC-P, IF/ICC, and ELISA applications.
• For Western blotting, the target protein has a predicted molecular weight of approximately 41 kDa; consider using stimulated or treated cell/tissue lysates where p38 MAPK phosphorylation is induced to enhance signal detection.
• In immunohistochemistry and immunofluorescence, antigen retrieval methods and careful control of phosphatase activity during sample preparation may be necessary to preserve the phospho-epitope. Include phosphatase inhibitor cocktails in lysis buffers and validate signal with phosphatase treatment controls.
• Cross-reactivity has been observed with human, mouse, and rat samples; optimization of antibody dilution and blocking conditions is recommended for each application and species to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing high-quality IVD raw materials for diagnostic and research applications. This anti-phospho-p38 MAPK (Y182) rabbit monoclonal antibody supports investigations into cellular stress, inflammation, and apoptosis pathways, and complements our broad portfolio of validated phospho-specific antibodies and ancillary reagents. With capabilities ranging from custom antibody development to bulk supply of matched antibody pairs and rare target sourcing, CamelBio streamlines your procurement process—helping you move from concept to clinic with confidence.