Anti-Phospho-p38 MAPK (Y182) Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - Q16539

Core Product Specifications and Parameters

Biological Background: p38 MAPK (MAPK14) Function and Localization

• MAPK14 is a serine/threonine kinase that serves as an essential component of the MAP kinase signal transduction pathway, mediating cellular responses to extracellular stimuli such as pro-inflammatory cytokines and physical stress.
• It phosphorylates and activates transcription factors (e.g., CREB1, ATF1, RELA, STAT1, STAT3) and histone H3 via downstream kinases MSK1 and MSK2, thereby inducing immediate-early genes in response to stress or mitogenic stimuli. Related references: PMID:9687510 PMID:9792677
• MAPK14 regulates protein synthesis by activating MKNK1/MNK1 and MKNK2/MNK2, which phosphorylate the initiation factor EIF4E2. Related references: PMID:11154262
• It modulates protein turnover through phosphorylation of the ubiquitin ligase SIAH2, controlling EGLN3 degradation, and also interferes with autophagy by affecting ATG9 trafficking. Related references: PMID:17003045 PMID:19893488
• In the cytoplasm, the p38 MAPK pathway is an important regulator of endocytosis; for example, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR and RAB5A effectors. Related references: PMID:16932740
• MAPK14 phosphorylates ADAM17, promoting ectodomain shedding of TGF-alpha family ligands and subsequent EGFR signaling activation. Related references: PMID:20188673
• The kinase phosphorylates CDC25B and CDC25C, leading to G2 delay after ultraviolet radiation through 14-3-3 protein binding. Related references: PMID:11333986
• MAPK14 localizes to both cytoplasm and nucleus, and is expressed in brain, heart, placenta, pancreas, and skeletal muscle, with lower levels in lung, liver, and kidney. Isoforms MXI2 and EXIP exhibit distinct activation and substrate specificities.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphopeptide corresponding to the Y182 phosphorylation site. To confirm phospho-specificity, consider treating samples with λ-phosphatase or using a non-phosphorylated peptide as a blocking control in parallel experiments.
• For Western blotting, use positive controls such as cell lysates from cultures stimulated with stress inducers (e.g., anisomycin, UV radiation, or pro-inflammatory cytokines) to induce robust p38 MAPK Y182 phosphorylation.
• When performing immunofluorescence, carefully optimize fixation and permeabilization conditions, as harsh treatments may compromise the phospho-epitope. Including a phosphatase inhibitor cocktail during sample preparation may help preserve the phosphorylation signal.
• The antibody is qualified for WB, IF/ICC, and ELISA. For other applications, additional validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This phospho-specific p38 MAPK antibody supports research and diagnostic development in inflammation, stress biology, and signal transduction. For rare targets or custom raw-material sourcing needs, CamelBio offers integrated solutions from concept to clinic.