Anti-Phospho-p38 MAPK (T180) Monoclonal Antibody for WB, IHC-P, ELISA - Q16539 / P53778 / Q15759

Core Product Specifications and Parameters

Biological Background: p38 MAPK (MAPK14) Function and Localization

• MAPK14 (p38 alpha) is a serine/threonine kinase and a core component of the MAP kinase signal transduction pathway, responding to stress stimuli and pro-inflammatory cytokines; it is one of four p38 MAPK family members with an estimated 200–300 substrates per kinase.
• The kinase rapidly activates downstream kinases including MAPKAPK2/MK2 and MAPKAPK3/MK3, which regulate gene expression post-transcriptionally by phosphorylating mRNA‑binding proteins such as ZFP36 and ELAVL1, and by modulating translation elongation factor EEF2K.
  Related references:
  PMID:11154262
• In the nucleus, p38 MAPK phosphorylates transcription factors (ATF1, ATF2, ATF6, ELK1, MEF2, p53, etc.) and chromatin remodelers, enabling rapid induction of immediate-early and inflammatory genes; phosphorylation of histone H3 (Ser‑10) by MSK1/2 facilitates NF‑κB recruitment.
  Related references:
  PMID:9687510
  PMID:9792677
  PMID:10330143
  PMID:9430721
  PMID:9858528
• The kinase shuttles between cytoplasm and nucleus and is involved in diverse cellular processes: it regulates protein turnover (e.g., CFLAR stability, SIAH2‑mediated EGLN3 degradation), autophagy (ATG9 trafficking), receptor endocytosis (EGFR via RAB5A effectors), and ectodomain shedding (ADAM17 phosphorylation).
  Related references:
  PMID:17003045
  PMID:19893488
  PMID:16932740
  PMID:20188673
• MAPK14 undergoes phosphorylation at Thr‑180 and Tyr‑182 within the activation loop, a dual event essential for kinase activity; additional post‑translational modifications include acetylation and ubiquitination, as indicated in UniProt keywords.
• The protein is widely expressed, with highest levels in brain, heart, placenta, pancreas, and skeletal muscle; lower expression is observed in lung, liver, and kidney.
• Alternative splicing generates isoforms such as MXI2 (responsive to mitogens and oxidative stress) and EXIP (implicated in early apoptosis onset) that exhibit differential substrate preferences and signaling outputs.
• In response to DNA damage, MAPK14 phosphorylates CDC25B/C leading to G2 arrest, and TIAR to stabilize GADD45A mRNA; it also activates the NLRP1 inflammasome after UV‑B or ribosome collisions.
  Related references:
  PMID:11333986
  PMID:20932473
  PMID:35857590

Experimental Guidance and Technical Tips

• The immunogen matches a phosphorylated peptide around Thr‑180; therefore, the antibody primarily detects activated (phosphorylated) p38 MAPK. For verification, consider treating samples with λ‑phosphatase or using unstimulated control lysates to confirm phospho‑specificity in Western blot.
• For immunohistochemistry (IHC‑P), microwave‑ or pressure‑cooker antigen retrieval may enhance detection of phospho‑epitopes; validate staining specificity by pre‑incubating the antibody with the immunizing phosphopeptide.
• ELISA applications can use the antibody as a capture or detection reagent for quantifying activated p38 in cell lysates; include recombinant phosphorylated p38 protein as a standard curve control if possible.
• When analyzing multiple p38 isoforms, note that this antibody likely cross‑reacts with p38 beta (MAPK11) and gamma (MAPK13) due to high sequence homology around Thr‑180; validate isoform specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated B2B partner for diagnostic manufacturers and research laboratories, CamelBio facilitates access to validated IVD antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For projects targeting cell signaling, inflammation, or stress‑response pathways, we can supply phospho‑specific antibodies, matched pairs, and rare‑target raw materials to streamline your assay development pipeline.