Anti-Phospho-Nucleolin-T76 Rabbit Monoclonal Antibody for IHC-P, ELISA - P19338

Core Product Specifications and Parameters

Biological Background: Nucleolin Function and Localization

• Nucleolin, also known as Protein C23, is the major nucleolar protein in growing eukaryotic cells and is encoded by the NCL gene.
• It binds histone H1 and induces chromatin decondensation, facilitating pre-rRNA transcription and ribosome assembly.
• Nucleolin also participates in transcriptional elongation and binds RNA oligonucleotides with 5'-UUAGGG-3' repeats.
• It is primarily localized in the nucleolus but also found in the nucleus and cytoplasm.
• Post-translational modifications include phosphorylation, acetylation, methylation, and ubiquitination, with phosphorylation at T76 being a key regulatory site.
• It is a DNA- and RNA-binding phosphoprotein involved in diverse nuclear processes.

Experimental Guidance and Technical Tips

• Since the immunogen is a phospho-peptide corresponding to T76, the antibody is specific for phosphorylated Nucleolin at this site. Verify specificity by comparing signals in phosphatase-treated versus untreated samples.
• For IHC-P, consider using appropriate antigen retrieval methods and validate nuclear/nucleolar staining patterns in human tissues known to express Nucleolin.
• ELISA detection may require coating with phospho-peptide or whole protein; consider optimizing coating buffer and blocking conditions.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio provides a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your diagnostic and research workflows.
• The nucleolin pathway and ribosome biogenesis research area can benefit from CamelBio’s one-stop procurement solutions, streamlining your supply chain from concept to clinic.