Anti-Phospho-NRF2-S40 Rabbit Monoclonal Antibody for WB - Q16236

Core Product Specifications and Parameters

Biological Background: NRF2 Function and Localization

• NRF2 (Nuclear factor erythroid 2-related factor 2), encoded by NFE2L2, is a transcription factor that regulates cellular responses to oxidative stress by binding to antioxidant response elements (AREs) in promoters of cytoprotective genes.
  Related references: PMID:11035812 PMID:19489739 PMID:29018201
• Under basal conditions, NRF2 is kept at low levels via KEAP1-mediated ubiquitination and proteasomal degradation in the cytoplasm.
  Related references: PMID:11035812 PMID:15601839 PMID:29018201
• Upon oxidative stress or electrophilic challenge, KEAP1 is inhibited, allowing NRF2 to translocate to the nucleus, dimerize with small Maf proteins, and activate ARE-driven gene transcription.
  Related references: PMID:19489739 PMID:29590092
• NRF2 also participates in selective autophagy, where the SQSTM1/p62-KEAP1 interaction leads to NFE2L2 stabilization and pathway activation.
  Related references: PMID:20452972
• Beyond redox regulation, NRF2 suppresses innate immune and inflammatory responses by blocking pro-inflammatory cytokine transcription and inhibiting IRF3 dimerization, and it restricts replication of viruses including SARS-CoV-2, HSV, and Zika virus.
  Related references: PMID:30158636 PMID:33009401
• NRF2 is widely expressed, with highest levels in adult muscle, kidney, lung, liver, and fetal muscle. Subcellularly, it shuttles between the cytoplasm and nucleus.
• Post-translational modifications include phosphorylation (e.g., at Ser-40), acetylation, glycation, and ubiquitination, which modulate its stability and activity.
• Alternative splicing generates multiple isoforms of NRF2.

Experimental Guidance and Technical Tips

• For Western blotting, consider using reducing conditions and loading appropriate protein amounts from cell lysates treated with oxidative stressors (e.g., H₂O₂) to detect NRF2 phosphorylation at Ser-40.
• For IHC-P, validate staining in NRF2-positive tissues such as liver or kidney, and include phosphatase inhibitor treatment during sample preparation.
• Cross-reactivity with mouse and rat has been reported; verify the expected band size (~68 kDa) in relevant species.
• In immunofluorescence, monitor nuclear translocation upon oxidative stress induction.
• For ELISA, use phosphorylated peptide controls to confirm specificity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting your NRF2-related oxidative stress and infectious disease research. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and can source rare-target raw materials to accelerate your assay development and manufacturing. Partner with us for seamless procurement across the entire diagnostic value chain.