Anti-Phospho-NRF2-S40 Rabbit mAb for WB, IF/ICC, IHC-P, ELISA - Q16236

Core Product Specifications and Parameters

Biological Background: NRF2 Function and Localization

• NRF2 (Nuclear factor erythroid 2-related factor 2) is a transcription factor crucial for oxidative stress response, binding to antioxidant response elements (ARE) and regulating cytoprotective genes (PubMed:11035812, PubMed:19489739, PubMed:29018201). Related references: PMID:11035812, PMID:19489739, PMID:29018201
• Under basal conditions, NRF2 is ubiquitinated and degraded via KEAP1-CUL3 complex, but oxidative stress inhibits KEAP1, leading to NRF2 nuclear accumulation and target gene activation (PubMed:15601839, PubMed:29590092). Related references: PMID:15601839, PMID:29590092
• The NRF2 pathway is also activated by selective autophagy via p62/SQSTM1 interaction, and during unfolded protein response (UPR), contributing to redox homeostasis (PubMed:20452972). Related references: PMID:20452972
• NRF2 regulates innate immune responses: it suppresses pro-inflammatory cytokine transcription, inhibits STING1 expression reducing antiviral DNA sensing, and limits release of pro-inflammatory cytokines during SARS-CoV-2 infection (PubMed:30158636, PubMed:33009401). Related references: PMID:30158636, PMID:33009401
• Subcellular localization: under normal conditions, predominantly cytoplasmic; upon activation, translocates to the nucleus.
• Widely expressed with highest levels in adult muscle, kidney, lung, liver, and fetal muscle.
• Post-translational modifications include phosphorylation, acetylation, glycation, and ubiquitination, regulating its activity and stability.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around Ser40; ensure samples are prepared with phosphatase inhibitors to preserve phosphorylation.
• For Western blotting, expect a band around 68 kDa. Verify specificity using phosphatase-treated lysates.
• In immunofluorescence, nuclear translocation of NRF2 can be induced by oxidative stressors (e.g., H2O2) – normalize with total NRF2 antibody.
• For IHC-P, antigen retrieval and antibody dilution should be optimized for the tissue of interest; validate with relevant controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides one-stop access to IVD raw materials, covering oxidative stress and cytoprotection research needs. We supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assist in sourcing rare targets like phospho-specific NRF2 antibodies. Partner with us to streamline your diagnostic development from concept to clinic.