Anti-Phospho-NPM1-T199 Rabbit Polyclonal Antibody for WB, ELISA - P06748

Core Product Specifications and Parameters

Biological Background: Nucleophosmin Function and Localization

• Nucleophosmin (NPM1, also known as B23, NO38, numatrin) is a nucleolar phosphoprotein essential for ribosome biogenesis, coordinating rRNA processing and ribosome nuclear export.
• Serves as a histone chaperone for core histones H3, H2B, and H4, facilitating proper chromatin assembly and nucleosome formation.
• Regulates centrosome duplication and centriole replication; phosphorylation by PLK2 triggers centriole replication.
• Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis; antagonizes ATF5-induced G2/M blockade to promote cell proliferation. Related references: PMID:22528486
• Interacts with MYC to enhance transcription of MYC target genes, potentially contributing to oncogenesis. Related references: PMID:25956029
• Localizes predominantly to the nucleolus, but also found in nucleoplasm, cytoplasm, and centrosomes, reflecting its multiple cellular functions.
• Subject to numerous post-translational modifications including phosphorylation (e.g., Thr199), acetylation, ADP-ribosylation, and ubiquitination, which modulate its activity and subcellular trafficking.
• Overexpression and mutations (e.g., cytoplasmic dislocation) are associated with acute myeloid leukemia and other cancers, highlighting its role as a proto-oncogene.

Experimental Guidance and Technical Tips

• This phospho-specific antibody was raised against a synthetic peptide phosphorylated at Thr199; for WB, include phosphatase inhibitors during sample preparation to preserve phosphorylation.
• Validate the antibody in your experimental system by comparing signals between untreated and phosphatase-treated lysates or using non-phosphorylated peptide blocking.
• For ELISA, coat with phosphorylated peptide to assess specificity, and consider cross-reactivity testing with non-phosphorylated NPM1.
• Since NPM1 shuttles to cytoplasm under certain conditions, fractionation or immunofluorescence could complement detection of phospho-NPM1 subcellular distribution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering a wide array of antibodies, antigens, and ancillary reagents to accelerate your diagnostic development. This phospho-specific NPM1 antibody supports research into nucleophosmin-related cellular processes and cancer biomarker studies, backed by our expert technical consultation and reliable supply chain.