Anti-Phospho-NMDAR1-S890 Rabbit Polyclonal Antibody for WB, ELISA - Q05586

Core Product Specifications and Parameters

Biological Background: NMDAR1 Function and Localization

• NMDAR1 (GluN1) is a mandatory subunit of NMDA receptors, which assemble as heterotetrameric, ligand-gated cation channels with high calcium permeability and voltage-dependent magnesium block. Related references: PMID:21376300, PMID:26875626, PMID:26919761
• These receptors are fundamental for synaptic plasticity, contributing to learning and memory by mediating long-term potentiation (LTP). Related references: PMID:26875626
• Channel activation requires glutamate binding to GluN2 subunits, glycine or D-serine binding to GluN1, and membrane depolarization to remove Mg²⁺ block. Related references: PMID:21376300, PMID:26875626, PMID:26919761
• NMDARs simultaneously conduct potassium efflux and sodium/calcium influx, with subunit composition (GluN2 or GluN3) dictating kinetic properties, pH sensitivity, and allosteric modulation. Related references: PMID:26875626, PMID:26919761, PMID:36309015
• NMDAR1 is predominantly localized to postsynaptic membranes, especially at excitatory synapses in the central nervous system.
• The protein is subject to extensive post-translational modification, including phosphorylation at multiple sites, which modulates receptor trafficking and signaling.
• Mutations in the GRIN1 gene are linked to neurological disorders such as epilepsy and intellectual disability, highlighting its critical role in brain function.

Experimental Guidance and Technical Tips

• This phospho-specific antibody was raised against a synthetic phosphopeptide around Ser890, enabling selective detection of phosphorylated NMDAR1. For specificity control, treat samples with phosphatase or use phospho-blocking peptide in your assay.
• In western blotting, expect a band at approximately 105 kDa. Use appropriate loading controls and validate with both phosphorylated and non-phosphorylated samples.
• The antibody is validated for WB and ELISA; for other applications like immunohistochemistry or immunoprecipitation, optimize and validate in the relevant sample system.
• Note cross-reactivity with human and rat NMDAR1. For use with other species, verify sequence homology at the phospho-site to ensure reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers, contract research organizations, and academic laboratories. For research on NMDA receptor phosphorylation and neurological disorders, we supply validated antibody pairs, bulk ancillary reagents, and tailored sourcing for rare targets, ensuring you have the critical resources needed from early-stage development to clinical application.