Anti-Phospho-NFKB1-S337 Rabbit mAb for WB, ELISA - P19838

Core Product Specifications and Parameters

Biological Background: NFKB1 Function and Localization

• NFKB1 encodes the p105 precursor protein, which is processed into the active p50 subunit of the NF-kappa-B transcription factor complex. The p105 form also retains NF-kappa-B proteins in the cytoplasm. Related references: PMID:1423592
• The NF-kappa-B complex is a pleiotropic transcription factor present in almost all cell types and regulates genes involved in inflammation, immunity, differentiation, cell growth, and apoptosis.
• The active p50 subunit forms heterodimers with RELA/p65 to create the p65-p50 complex, a major transcriptional activator that binds to the kappa-B consensus sequence in target gene enhancers. Related references: PMID:1740106, PMID:7830764
• p50-p50 homodimers act as transcriptional repressors, but can become activators when associated with BCL3.
• NFKB1/p105 also represses MAP3K8-induced MAPK signaling until proteasome-dependent degradation releases active MAP3K8.
• Subcellular localization alternates between cytoplasm (inactive, bound to inhibitors) and nucleus (active, after release from I-kappa-B).
• Phosphorylation at serine 337 is one of the multiple post-translational modifications that regulate NFKB1 activity, processing, or interactions; this antibody specifically recognizes the phosphorylated form at that site.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide around S337; confirm specificity by using phosphatase-treated samples or competing with non-phosphorylated peptide.
• For Western blot, consider using samples from cells stimulated to activate NF-kappa-B signaling (e.g., with TNF-α) to observe phospho-specific band shifts at 85kDa/105kDa.
• ELISA detection may be optimized by pairing with a total NFKB1 antibody or using phospho-specific detection.
• Validate in human cell lines or tissues with known NF-kappa-B activation; cross-reactivity with other species is not guaranteed.
• Always include appropriate negative and positive controls to distinguish phosphorylation-dependent signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers a comprehensive suite of IVD raw materials, supporting diagnostic manufacturers and labs from concept to clinic. This phosphorylated NFKB1 antibody fits into inflammation and immunology research portfolios, and we can source validated antibody pairs, bulk ancillary reagents, and rare-target raw materials to accelerate your assay development. Partner with CamelBio for reliable, one-stop procurement of cutting-edge diagnostic reagents.