Anti-Phospho-NF-κB2-S866 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q00653

Core Product Specifications and Parameters

Biological Background: NF-κB2 p100/p52 Function and Localization

• NF-κB2 encodes the p100 precursor protein, which is proteolytically processed to generate the active p52 subunit; isoform p49 is also expressed. p100 can retain NF-κB complexes in the cytoplasm.
• NF-κB is a pleiotropic transcription factor that regulates inflammation, immunity, cell differentiation, growth, tumorigenesis, and apoptosis.
• p52 forms homo- or heterodimers with other NF-κB/Rel family members (RELA/p65, RELB, NFKB1/p50, REL) and binds to the κB consensus sequence 5'-GGRNNYYCC-3' in target gene enhancers.
• The heterodimeric RelB-p52 complex functions as a transcriptional activator, while the p52-p52 homodimer acts as a repressor.
• In the non-canonical NF-κB pathway, MAP3K14-activated CHUK/IKKA homodimer phosphorylates p100 associated with RelB, triggering ubiquitin-dependent processing to p52 and nuclear translocation.
• NF-κB2 is localized in both the cytoplasm and nucleus, with subcellular compartmentalization and post-translational modifications (phosphorylation, ubiquitination) tightly controlling its activity.
• p52, in concert with RELB, regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-BMAL1 heterodimer.
• Chromosomal rearrangements involving NFKB2 are associated with certain lymphoid malignancies.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide encompassing Ser866; thus, the antibody is expected to specifically recognize NF-κB2 only when phosphorylated at this residue. Use phosphatase inhibitors during sample preparation to preserve phosphorylation.
• For Western blotting, the predicted molecular weight of p100 is ~97 kDa; however, multiple bands may be observed due to processing intermediates or degradation. Including appropriate positive and negative controls (e.g., phosphatase-treated lysates) is recommended.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimization of antigen retrieval, permeabilization, and blocking steps may be necessary. The antibody's performance should be validated in the relevant tissue or cell system.
• In ELISA applications, the phospho-specific antibody can be paired with a capture antibody recognizing total NF-κB2 to quantify phosphorylated protein levels.
• Cross-reactivity with mouse is reported, but reactivity with other species has not been tested; further validation is advised if using non-human/mouse samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw-material sourcing, with expertise spanning the NF-κB signaling and inflammation research areas. We offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and efficient sourcing of rare-target raw materials. Whether you need this Phospho-NF-κB2-S866 antibody for assay development or other critical IVD components, CamelBio streamlines your procurement from concept to clinic.