Anti-Phospho-NF-kB p65/RelA (Ser529) Rabbit mAb for WB, ELISA - Q04206

Core Product Specifications and Parameters

Biological Background: NF-kB p65/RelA Function and Localization

• NF-kB p65/RelA is a subunit of the NF-kappa-B transcription factor complex, which forms various homo- and heterodimers; the RELA-NFKB1 heterodimer is the most abundant.
• NF-kB regulates a wide range of biological processes such as inflammation, immunity, cell differentiation, and apoptosis.
• In the inactive state, NF-kB is retained in the cytoplasm by I-kappa-B inhibitor proteins; upon stimulation, I-kappa-B is phosphorylated and degraded, enabling NF-kB nuclear translocation.
• Phosphorylation at specific residues, including Ser529, is a crucial post-translational modification that modulates RelA transcriptional activity, DNA binding, and interaction with cofactors.
• RelA is essential for cytokine gene expression in T-cells.
  Related references:
  PMID:15790681
• RelA functions as a key transcription factor regulating the interferon response during SARS-CoV-2 infection.
  Related references:
  PMID:33440148
• Subcellularly, RelA shuttles between the cytoplasm and nucleus, a process tightly controlled by phosphorylation and inhibitor binding.
• It is subject to multiple post-translational modifications including phosphorylation, acetylation, methylation, and ubiquitination, which fine-tune its activity.

Experimental Guidance and Technical Tips

• As a phospho-specific antibody, it is recommended to treat samples with phosphatase inhibitors during preparation and to verify the specificity of the signal by dephosphorylation controls or treatment with relevant stimuli.
• For Western blotting, a band at approximately 60 kDa is expected for full-length RelA; however, the apparent molecular weight may shift due to multiple phosphorylation events.
• For ELISA applications, the use of an immunogen peptide or a phosphorylated protein standard can aid in optimizing coating concentrations and detection sensitivity.
• Cross-reactivity testing with non-human species may be warranted if the target sequence is conserved, but validation data are only available for human samples.
• Always titrate the antibody in your specific experimental conditions to determine optimal working dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This phospho-NF-kB p65 (Ser529) rabbit monoclonal antibody supports research into NF-κB-mediated inflammation, immunity, and oncogenesis. We supply validated antibody pairs, optimized monoclonals, bulk ancillary reagents, and rare-target raw material sourcing to accelerate your assay development and diagnostic workflows.