Anti-Phospho-NF-kB p65/RelA (S536) Monoclonal Antibody for WB, IF/ICC, ELISA - Q04206

Core Product Specifications and Parameters

Biological Background: NF-κB p65/RelA Function and Localization

• RELA encodes transcription factor p65 (also known as nuclear factor NF-kappa-B p65 subunit), a central subunit of the NF-κB complex.
• NF-κB is a pleiotropic transcription factor that controls genes in inflammation, immunity, cell growth, differentiation, tumorigenesis, and apoptosis, activated by various stimuli.
• p65 forms homo- or heterodimers with other Rel proteins; the RELA-NFKB1 heterodimer is the most abundant and activates transcription.
• In resting cells, NF-κB is retained in the cytoplasm by IκB inhibitors; upon activation, IκB is degraded, freeing NF-κB to translocate to the nucleus and bind DNA.
• Phosphorylation at serine 536 (S536) in the transactivation domain enhances transcriptional activation and serves as a marker of NF-κB pathway activation.
• p65 localizes to both nucleus and cytoplasm, shuttling in response to signals.
• p65 undergoes multiple post-translational modifications (phosphorylation, acetylation, methylation, ubiquitination, S-nitrosylation) that regulate its function.
• p65 is essential for cytokine gene expression in T-cells and regulates the interferon response during SARS-CoV-2 infection. Related references: PMID:15790681, PMID:33440148

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around S536; consider using a non-phosphopeptide control to confirm phospho-specificity in your assay.
• For western blot, a major band around 65 kDa is expected; validate detection in stimulated versus unstimulated cell lysates to confirm activation-dependent phosphorylation.
• For immunofluorescence/ICC, include appropriate permeabilization and consider co-staining with a total p65 antibody or nuclear marker to visualize translocation.
• ELISA protocols may utilize this antibody for capturing phospho-p65; optimize coating and detection conditions for the specific sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw-material sourcing, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents. This phospho-NF-κB p65 antibody supports research into inflammation, immunity, and infectious disease pathways. Let us streamline your supply chain, from concept to clinic, with bulk and rare-target procurement solutions.