Anti-Phospho-NDRG1 (T346) Rabbit Polyclonal Antibody for WB and ELISA - Q92597

Core Product Specifications and Parameters

Biological Background: NDRG1 Function and Localization

• NDRG1 (N-myc downstream-regulated gene 1) is a stress-responsive protein involved in hormone responses, cell growth, and differentiation.
• Functions as a tumor suppressor in many cell types and is required for p53/TP53-mediated caspase activation and apoptosis.
• Plays essential roles in cell trafficking, particularly in Schwann cells, and is necessary for peripheral nerve myelin sheath maintenance.
• Involved in vesicular recycling of CDH1 and TF, and may also function in lipid trafficking; protects cells from spindle disruption damage and regulates microtubule dynamics.
• Localizes to the cytoplasm, centrosome, nucleus, and cell membrane, consistent with its diverse functions.
• Post-translationally modified by phosphorylation and acetylation; associated with Charcot-Marie-Tooth disease.
• Ubiquitously expressed, with highest levels in placental membranes, prostate, kidney, small intestine, and ovary; reduced in adenocarcinomas.

Experimental Guidance and Technical Tips

• For Western blot, use a positive control lysate known to express phosphorylated NDRG1 (T346). Phosphatase treatment can confirm specificity for the phosphorylated form.
• In ELISA, consider using a capture antibody paired with this detection antibody to improve sensitivity; optimize coating conditions and antibody concentrations.
• The immunogen is a synthetic phosphorylated peptide; cross-reactivity with non-phosphorylated NDRG1 or other proteins should be evaluated in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with integrated access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents. For NDRG1-focused cancer or neurodegeneration research, we support procurement of rare-target antibodies and bulk reagents to streamline your assay development from concept to clinic.