Anti-Phospho-NCF4 (p40-phox) T154 Rabbit Polyclonal Antibody for WB and ELISA - Q15080

Core Product Specifications and Parameters

Biological Background: NCF4/p40-phox Function and Localization

• NCF4 encodes neutrophil cytosol factor 4, also known as p40-phox, an essential regulatory subunit of the phagocyte NADPH oxidase complex.
• Together with other cytosolic factors, p40-phox participates in the assembly and activation of the NOX2 enzyme complex, which generates superoxide anions for microbial killing.
• The protein contains an N-terminal PX domain that binds phosphatidylinositol 3-phosphate, targeting the complex to phagosomal and endosomal membranes.
• A central SH3 domain mediates interactions with other oxidase subunits, facilitating complex assembly.
• Phosphorylation at Thr154 is critical for activation, promoting conformational changes and membrane translocation.
• Expressed exclusively in hematopoietic cells, NCF4 plays a specialized role in innate immune defense.
• Mutations in NCF4 are associated with chronic granulomatous disease (CGD), characterized by recurrent infections due to defective superoxide production.

Experimental Guidance and Technical Tips

• Verify phospho-specificity by pre-incubating the antibody with non-phosphorylated peptide or treating cell lysates with phosphatase; loss of signal confirms phosphorylation dependence.
• For Western blot, optimize antibody dilution using a positive control such as phorbol ester-stimulated neutrophil or HL-60 cell lysates; include phosphatase inhibitors in lysis buffers to preserve phosphorylation.
• In ELISA, coat with the phosphorylated peptide immunogen and compare binding to the non-phosphorylated counterpart to assess specificity.
• Consider membrane stripping and reprobing with a total NCF4 antibody to normalize phosphorylation levels to protein abundance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner for sourcing high-quality IVD raw materials. We offer a comprehensive range of validated antibodies, including phospho-specific reagents like this anti-phospho-NCF4 antibody, to accelerate your diagnostic and research programs in innate immunity and oxidative stress. Our supply network ensures reliable access to optimized antibody pairs, bulk ancillary reagents, and assistance with rare target sourcing—supporting your journey from concept to clinic.