Anti-Phospho-mTOR (Ser2481) Rabbit Monoclonal Antibody for WB, ELISA - P42345

Core Product Specifications and Parameters

Biological Background: mTOR Function and Localization

• Serine/threonine-protein kinase that acts as a central regulator of cellular metabolism, growth, and survival in response to hormones, growth factors, nutrients, energy, and stress signals. Related references: PMID:12087098, PMID:12150925, PMID:12150926
• Functions as part of two distinct complexes, mTORC1 and mTORC2, each with unique downstream targets and regulatory mechanisms. Related references: PMID:15268862, PMID:15467718, PMID:18497260
• mTORC1 responds to nutrient and amino acid sufficiency, promoting protein synthesis via phosphorylation of EIF4EBP1 and RPS6KB1, and stimulating lipogenesis and nucleotide biosynthesis. Related references: PMID:12087098, PMID:12150925, PMID:12150926, PMID:23429703, PMID:23429704, PMID:23426360
• mTORC2 transduces growth factor signals to control proliferation, cytoskeletal organization, and anabolic output, primarily by phosphorylating AGC kinases such as AKT, PKC, and SGK1. Related references: PMID:15268862, PMID:15467718, PMID:21376236
• Subcellular localization includes lysosome, endoplasmic reticulum, Golgi, mitochondrial outer membrane, cytoplasm, nucleus, and phagosomes, reflecting its diverse functional contexts.
• Expressed in numerous tissues, with highest levels in testis.
• This antibody was raised against a synthetic phosphopeptide corresponding to residues surrounding Ser2481 of human mTOR, enabling specific detection of phosphorylation at this site.
• MTOR is subject to various post-translational modifications including phosphorylation, acetylation, and ubiquitination, as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• For Western blot, due to the high molecular weight of mTOR (289 kDa), use a low percentage polyacrylamide gel (e.g., 6–8%) and optimize transfer conditions to achieve efficient membrane transfer.
• To maintain phosphorylation at Ser2481, include phosphatase inhibitors in cell lysis buffers and avoid prolonged sample handling.
• The antibody has been validated for WB and ELISA with human and mouse samples; cross-reactivity with other species should be determined empirically.
• For optimal results, store the antibody as recommended and minimize freeze-thaw cycles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. With expertise in phospho-specific antibodies and cell signaling targets like mTOR, we assist diagnostic manufacturers and researchers in streamlining their raw material procurement from concept to clinic.