Anti-Phospho-mTOR-S2448 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P42345

Core Product Specifications and Parameters

Biological Background: Serine/threonine-protein kinase mTOR Function and Localization

• Serine/threonine-protein kinase mTOR (MTOR, also known as FRAP/FRAP1/FRAP2/RAFT1/RAPT1) is a 289 kDa master regulator of cellular metabolism, growth, and survival, integrating hormonal, nutrient, and stress signals. Related references: PMID:12087098 PMID:12150925 PMID:12150926
• MTOR functions as the catalytic core of two distinct complexes, mTORC1 and mTORC2, which differ in subunit composition, upstream regulation, and downstream targets. Related references: PMID:15268862 PMID:15467718 PMID:18497260
• Activated mTORC1 promotes protein synthesis by phosphorylating EIF4EBP1 to relieve its inhibition of eIF4E, and by phosphorylating RPS6KB1/2 to drive ribosomal protein S6 phosphorylation and translation initiation. Related references: PMID:12087098 PMID:12150925 PMID:12150926 PMID:24403073 PMID:29236692 PMID:18925875
• mTORC1 stimulates lipid and nucleotide synthesis by activating SREBF1/SREBP1 and CAD, respectively, coordinating macromolecule production for cell growth. Related references: PMID:23429703 PMID:23429704 PMID:23426360
• Under nutrient sufficiency, mTORC1 suppresses autophagy by phosphorylating key autophagy regulators including ULK1 (Ser‑758), DAP, RUBCNL, and AMBRA1. Related references: PMID:32561715 PMID:20537536 PMID:30704899 PMID:23524951 PMID:25438055
• The mTORC2 complex phosphorylates AGC kinases including AKT at Ser‑473, PKC, and SGK1, regulating cell survival, proliferation, and actin cytoskeleton reorganization. Related references: PMID:15268862 PMID:15467718 PMID:21376236 PMID:15718470 PMID:24670654
• MTOR dynamically localizes to lysosomal, endoplasmic reticulum, Golgi, mitochondrial, and nuclear membranes; mTORC1 activation is tightly coupled to its recruitment to the lysosomal surface.
• MTOR is broadly expressed, with highest levels in testis, and is regulated by extensive phosphorylation, acetylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide spanning Ser‑2448; therefore, the antibody recognizes mTOR only when phosphorylated at this residue. For Western blot, treat cells with appropriate stimuli (e.g., growth factors, amino acids) and include phosphatase inhibitors in lysis buffers to preserve phosphorylation.
• For Western blotting of the 289 kDa mTOR protein, use a low‑percentage polyacrylamide gel (e.g., 6–8%) and a longer transfer time. Verify transfer efficiency with a pre‑stained marker.
• For immunofluorescence (IF/ICC), fix cells with paraformaldehyde, permeabilize appropriately, and use a blocking buffer to reduce non‑specific binding. Confirm localization by co‑staining with lysosomal markers if studying mTORC1 activation.
• ELISA applications may involve coating with peptide or protein; validate assay conditions (coating buffer, blocking agent, antibody dilution) in your specific matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one-stop IVD raw-material sourcing partner, providing high-quality antibodies and critical reagents for every stage of diagnostic development. This phospho‑mTOR‑S2448 polyclonal antibody exemplifies our commitment to supplying validated tools for cell signaling and oncology research. Beyond individual antibodies, we offer optimized antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets to streamline your assay development and manufacturing pipelines.