Anti-Phospho-mTOR-S2448 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P42345

Core Product Specifications and Parameters

Biological Background: mTOR Function and Localization

• Serine/threonine-protein kinase mTOR (also known as FRAP, RAFT1, RAPT1) serves as a central regulator of cellular metabolism, growth, and survival, integrating signals from hormones, growth factors, nutrients, energy, and stress. Related references: PMID:12087098, PMID:12150925, PMID:12150926

• MTOR functions within two distinct complexes, mTORC1 and mTORC2, which control anabolic and cytoskeletal processes, respectively. Related references: PMID:15268862, PMID:15467718, PMID:18497260

• Through mTORC1, it promotes protein synthesis by phosphorylating translation regulators (e.g., EIF4EBP1, RPS6KB1/RPS6KB2) and enhances lipid and nucleotide synthesis (via SREBF1, CAD, and pentose phosphate pathway). Related references: PMID:12087098, PMID:12150925, PMID:12150926, PMID:24403073, PMID:29236692, PMID:18925875, PMID:23429703, PMID:23429704

• mTORC1 also inhibits catabolic pathways, primarily autophagy, by phosphorylating ULK1, DAP, RUBCNL, and AMBRA1 under nutrient-rich conditions. Related references: PMID:32561715, PMID:20537536, PMID:30704899, PMID:23524951, PMID:25438055

• The mTORC2 complex mediates growth factor signaling by activating AGC kinases (AKT, PKC, SGK1) and regulating the actin cytoskeleton and insulin signaling. Related references: PMID:15268862, PMID:15467718, PMID:21376236, PMID:15718470, PMID:24670654, PMID:18925875

• mTOR localizes to multiple cellular membranes, including the lysosome (for mTORC1 activation), as well as the endoplasmic reticulum, Golgi, mitochondria, and plasma membrane; it also shuttles to the nucleus.

• Expressed in many tissues with highest levels in testis.

Experimental Guidance and Technical Tips

• This antibody specifically recognizes phosphorylation at Ser2448 of mTOR; consider including phosphatase inhibitors in lysis buffers for immunoblotting.
• For Western blot (WB), validate the antibody in your sample system under conditions that activate mTOR (e.g., growth factor stimulation) to confirm phospho-specificity.
• In immunofluorescence (IF/ICC), optimize fixation and permeabilization protocols to preserve the phospho-epitope while achieving adequate antibody access.
• Since mTOR is a large protein (289 kDa), use appropriate gel percentages (e.g., 6–8% SDS-PAGE) and extended transfer times for blotting.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a dedicated one-stop partner for IVD raw materials, technical support, and consulting from concept to clinic. Alongside this anti-phospho-mTOR-S2448 antibody, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and access to rare target raw materials for mTOR signaling and cancer research. Let us help streamline your assay development and supply chain with reliable, traceable bioreagents.