Anti-Phospho-MKK3 (S189) + MKK6 (S207) Rabbit Monoclonal Antibody for WB/IF/ELISA - P46734 / P52564

Core Product Specifications and Parameters

Biological Background: MKK3 and MKK6 Function and Localization

• MKK3 (MAP kinase kinase 3), also known as MEK3 or SAPKK2, is encoded by the MAP2K3 gene and belongs to the dual specificity protein kinase family. It is closely related to MKK6 (encoded by MAP2K6), with both proteins sharing high homology and functioning as key activators of p38 MAP kinases.
• As a dual specificity kinase, MKK3 catalyzes the concomitant phosphorylation of threonine and tyrosine residues in the activation loop of p38 MAP kinase isoforms, a critical step in stress-responsive signaling.
• MKK3 is activated by various environmental stresses and proinflammatory cytokines in vivo, linking extracellular signals to intracellular responses such as inflammation, differentiation, and apoptosis.
• It operates within a signal transduction pathway initiated by the adrenergic receptor ADRA1B, leading to the activation of MAPK14 (p38α), highlighting its role in G protein-coupled receptor signaling.
• At the tissue level, MKK3 shows abundant expression in skeletal muscle and is widely expressed across other tissues, suggesting broad involvement in stress and metabolic regulation.
• Post-translational modifications of MKK3 include acetylation and phosphorylation; the S189 residue is a critical activation site. The protein also undergoes alternative splicing producing multiple isoforms.
• MKK3 is associated with disease variants, and its dysregulation has been implicated in pathological conditions, underscoring its importance as a therapeutic target.

Experimental Guidance and Technical Tips

• Because this antibody recognizes phosphorylation at S189 of MKK3 and S207 of MKK6, use phosphatase inhibitors during cell lysis and sample preparation to preserve phosphorylation signals.
• For Western blot, consider loading positive controls treated with stress inducers (e.g., anisomycin, UV, or osmotic shock) to verify antibody reactivity against the activated forms.
• In immunofluorescence, validate the antibody in relevant cell lines under stimulated versus unstimulated conditions to confirm specific phospho-signal localization.
• Multiple bands corresponding to different isoforms and splice variants (reported molecular weights around 36–39 kDa for MKK3 and 31–37 kDa for MKK6) may be observed; consult the specific protein weights and ensure proper molecular weight markers.

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CamelBio provides diagnostic manufacturers and research laboratories with integrated access to premium IVD raw materials, including phospho-specific antibodies like this anti-phospho-MKK3/MKK6 clone. Our portfolio covers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, enabling streamlined development of stress kinase pathway assays. For rare or custom targets, we offer expert sourcing and supply chain solutions to support your projects from concept to clinic.