Anti-Phospho-MEK2 (T394) Rabbit Polyclonal Antibody for WB and IF/ICC - P36507

Core Product Specifications and Parameters

Biological Background: MEK2 Function and Localization

• MEK2 (MAP2K2) is a dual specificity mitogen-activated protein kinase kinase that catalyzes the phosphorylation of both threonine and tyrosine residues in the Thr-Glu-Tyr motif of downstream MAP kinases, including ERK1 and ERK2.
• It functions as a central component of the MAPK/ERK signaling cascade, directly activating ERK1 and ERK2 in response to upstream stimuli, thereby regulating cell proliferation, differentiation, and survival.
• MEK2 facilitates BRAF activation by binding to KSR1 or KSR2, which releases inhibitory intramolecular interactions and promotes KSR-BRAF dimerization, enhancing signal propagation. Related references: PMID:29433126
• The protein localizes predominantly in the cytoplasm and at the membrane, consistent with its role in transmitting extracellular signals to intracellular effectors.
• Phosphorylation at Thr-394 (the epitope targeted by this antibody) is a critical regulatory post-translational modification; MEK2 is also subject to acetylation, influencing its activity and interactions.
• MEK2 contains an ATP-binding pocket and requires magnesium ions for catalytic activity, classifying it as a serine/threonine and tyrosine dual-specificity kinase.
• Mutations in MAP2K2 have been implicated in human diseases, including cardiomyopathy, ectodermal dysplasia, and intellectual disability, underscoring its biological significance.
• The predicted molecular weight of MEK2 is approximately 44 kDa, aligning with the expected band observed in Western blot analyses.

Experimental Guidance and Technical Tips

• The immunogen was a phospho-specific peptide, ensuring reactivity only with the phosphorylated T394 form; validate specificity by treating lysates with lambda phosphatase or by peptide competition assays.
• For Western blot, a band around 44 kDa should be detected in stimulated human cell lines; include both untreated and growth-factor-treated positive controls to confirm phospho-dependence.
• When performing IF/ICC, optimize fixation and permeabilization conditions, and consider co-staining with a total MEK2 antibody to distinguish phosphorylation states.
• Cross-reactivity with other species has not been reported; researchers should validate the antibody in their model system if using non-human samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, offering this anti-Phospho-MEK2 (T394) antibody alongside validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our portfolio supports MAPK/ERK pathway research and diagnostic development, ensuring seamless transition from discovery to clinical application. For rare target raw-material sourcing or customized technical support, CamelBio acts as your dedicated partner from concept to clinic.