Anti-Phospho-MEK1-S298 Rabbit Polyclonal Antibody for WB, IP, ELISA - Q02750

Core Product Specifications and Parameters

Biological Background: MEK1 Function and Localization

• MEK1, encoded by MAP2K1, is a dual specificity mitogen-activated protein kinase kinase that phosphorylates both threonine and tyrosine residues on its substrates, primarily ERK1/2.
• As a core component of the MAPK/ERK signaling cascade, MEK1 transduces signals from activated RAS/RAF to ERK, regulating cell growth, adhesion, survival, and differentiation.
• Upon activation, MEK1 catalyzes the concomitant phosphorylation of Thr and Tyr in the TEY motif of ERK1 and ERK2, leading to their activation and downstream transcriptional responses.
• In a KSR1 or KSR2-dependent manner, MEK1 releases the inhibitory intramolecular interaction of KSR proteins, promoting KSR-BRAF dimerization and BRAF activation. Related references: PMID:29433126
• MEK1 localizes to the cytoplasm, centrosome, spindle pole body, and can shuttle to the nucleus; it also associates with membranes, reflecting dynamic regulation during cell cycle and signaling.
• Widely expressed across tissues, with extremely low levels in brain, indicating its broad role in non-neuronal cell mitogenic signaling.
• Post-translational modifications include phosphorylation at multiple sites (e.g., S298) and acetylation, which modulate its activity and interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Ser298; thus, the antibody is expected to specifically recognize MEK1 phosphorylated at S298.
• For Western blot detection, it is advisable to include phosphatase inhibitors in the lysis buffer to preserve phosphorylation signals.
• This antibody may be applied in immunoprecipitation and ELISA; assess suitability for your experimental system with appropriate controls.
• Cross-reactivity with human, mouse, and rat has been indicated; however, always verify by comparing with untreated or phosphatase-treated samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for diagnostic manufacturers and research labs, supplying high-quality IVD raw materials, including phospho-specific antibodies like this anti-Phospho-MEK1-S298 antibody. We offer a wide range of validated antibody pairs, recombinant proteins, and ancillary reagents for MAPK pathway research and clinical assay development. Inquire about bulk orders or custom sourcing solutions for your next project.