Anti-Phospho-MEK1-S298 Monoclonal Antibody for WB - Q02750

Core Product Specifications and Parameters

Biological Background: MEK1 Function and Localization

• Also known as MAP2K1, MEK1 is a dual specificity mitogen-activated protein kinase kinase that catalyzes the concomitant phosphorylation of threonine and tyrosine residues on ERK1 and ERK2, serving as a key node in the MAPK/ERK signaling cascade.
• Acts downstream of RAF1 activation upon growth factor, cytokine, or hormone stimulation, relaying proliferative and differentiative signals through the RAS-RAF-MEK-ERK axis.
• Mediates BRAF activation in a KSR1- or KSR2-dependent manner; binding to these scaffold proteins releases autoinhibition, promoting KSR-BRAF dimerization and BRAF kinase activity. Related references: PMID:29433126
• Participates in diverse cellular processes including growth, adhesion, survival, and differentiation, largely via transcriptional regulation, metabolic modulation, and cytoskeletal reorganization.
• Regulates peroxisome proliferator-activated receptor gamma (PPARG) nuclear export, linking the MAPK pathway to metabolic and apoptotic control.
• Involved in endosomal dynamics, lysosome processing, perinuclear recycling compartment (PNRC) cycling, and Golgi fragmentation during mitosis.
• Localizes to the cytoplasm, centrosome, spindle pole body, nucleus, and membrane, reflecting dynamic roles in signaling and cell cycle control.
• Widely expressed across tissues with extremely low levels in the brain; classified as a phosphoprotein, a disease-variant-containing kinase, and a reference proteome member.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide spanning the S298 phosphorylation site; specificity for the phosphorylated form should be validated in systems where MEK1 S298 phosphorylation is induced (e.g., growth factor stimulation).
• For Western Blotting, the predicted molecular weight is 43 kDa; ensure adequate SDS-PAGE separation and include phosphatase inhibitors in lysis buffers to preserve the phospho-epitope.
• ELISA protocols can be adapted for quantitative detection of phospho-MEK1 in cell or tissue lysates; verify linearity and sensitivity in the intended sample matrix.
• Cross-reactivity is reported for human and rat; if testing other species or phospho-mimicry effects, confirm specificity using dephosphorylated or mutant controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw-material partner, streamlining procurement for diagnostic manufacturers and research labs working on the MAP kinase pathway. We offer validated antibody pairs, optimized rabbit monoclonal antibodies like this anti-Phospho-MEK1-S298 clone, bulk ancillary reagents, and specialized sourcing for rare targets. Our supply solutions support every project phase, from early-stage target validation to clinical-scale production.