Anti-Phospho-MARCKS-S158 Rabbit Polyclonal Antibody for WB - P29966

Core Product Specifications and Parameters

Biological Background: MARCKS Function and Localization

• Myristoylated alanine-rich C-kinase substrate (MARCKS) is a major PKC substrate that regulates actin cytoskeleton dynamics, cell motility, adhesion, phagocytosis, and exocytosis via membrane phospholipid sequestration (particularly PIP2) and protein docking (PubMed:23704996, PubMed:36009319). Related references: PMID:23704996, PMID:36009319
• In quiescent cells, MARCKS sequesters PIP2 at lipid rafts, inhibiting exocytosis; PKC phosphorylation reverses this regulation, linking kinase signaling to membrane trafficking (PubMed:23704996). Related references: PMID:23704996
• MARCKS promotes inflammatory cell migration, adhesion, and cytokine secretion (e.g., TNF) in macrophages, and mediates bacteria-induced ROS production in monocytes (PubMed:37949888). Related references: PMID:37949888
• The protein participates in neurite initiation, outgrowth, and axon development by interacting with CDC42 and RAB10-positive vesicle fusion at the growth cone (By similarity).
• MARCKS is detected in spermatozoa and is widely expressed; its subcellular localization spans the plasma membrane, cytoplasm, and cytoskeleton, reflecting dynamic intracellular shuttling.
• Post-translational modifications include N-terminal myristoylation (membrane anchoring), phosphorylation at Ser158 (PKC target), and acetylation, all critical for function and localization.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide corresponding to residues surrounding Ser158. For WB, use standard phospho-protein handling: include phosphatase inhibitors in lysis buffers and avoid excessive stripping/reprobing.
• Validate antibody specificity by treating samples with alkaline phosphatase or using non-phosphorylated peptide blocking to confirm phospho-dependent signal.
• Expected band: ~32 kDa, but note that MARCKS can appear higher due to post-translational modifications and anomalous migration.
• For species cross-reactivity, the antibody is reported to detect human, mouse, and rat; verify in your model system with appropriate positive controls (e.g., PKC-stimulated cells).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted IVD raw-material supplier offering this phospho-specific MARCKS antibody alongside validated antibody pairs, monoclonal/polyclonal options, and bulk ancillary reagents for signal transduction and cytoskeleton research. Our one-stop platform supports your procurement needs from early-stage assay development to clinical production, including sourcing of rare-target antibodies and custom services.