Anti-Phospho-MAPKAPK2 (T334) Rabbit Polyclonal Antibody for WB, ELISA - P49137

Core Product Specifications and Parameters

Biological Background: MAPKAPK2 (MK2) Function and Localization

• MAPKAPK2 is a stress-activated serine/threonine kinase that phosphorylates numerous substrates involved in cytokine production, endocytosis, cytoskeletal reorganization, cell migration, DNA damage response, and transcriptional regulation.
• It mediates phosphorylation of HSF1, promoting interaction with HSP90 and thereby inhibiting HSF1 homotrimerization, DNA-binding, and transactivation, which modulates the heat shock response.
  Related references: PMID:16278218
• In inflammatory signaling, MAPKAPK2 post-transcriptionally regulates TNF and IL6 production by phosphorylating AU-rich element (ARE)-binding proteins ELAVL1, HNRNPA0, PABPC1, and TTP/ZFP36, affecting cytokine mRNA stability and translation.
• The kinase participates in the DNA damage pathway by relocalizing from the nucleus to cytoplasm upon genotoxic stress and phosphorylating HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA and contributing to the G2/M checkpoint.
• Under UV-induced stress, MAPKAPK2 phosphorylates CEP131, which enhances its binding to 14-3-3 proteins and inhibits the formation of new centriolar satellites, linking it to centriole biology.
  Related references: PMID:26616734
• MAPKAPK2 is ubiquitously expressed in human tissues and localizes to both the cytoplasm and nucleus, with its subcellular distribution being crucial for functional specificity.
• It is classified under keywords including ATP-binding, kinase, serine/threonine-protein kinase, phosphoprotein, and Ubl conjugation pathway, reflecting its regulatory roles.

Experimental Guidance and Technical Tips

• This antibody specifically recognizes phosphorylation at threonine 334 (T334) of human MAPKAPK2. To detect the phospho-signal, stimulate cells with stress inducers (e.g., anisomycin, UV) and include phosphatase inhibitors in lysis buffers.
• For Western blot analysis, validate the specificity by comparing signals with and without phosphatase treatment, or by peptide competition using the phosphorylated versus non-phosphorylated immunogen peptide.
• While the antibody is recommended for WB and ELISA, users expanding to other applications (e.g., IHC, IF) should optimize conditions and confirm cross-reactivity with their sample species.
• Given its cross-reactivity with human, mouse, and rat, this antibody can be employed across common model organisms; however, always run appropriate controls to confirm species reactivity in your experimental system.
• For ELISA-based assays, consider optimizing coating concentrations and detection antibodies to achieve the best signal-to-noise ratio.
• As with any phospho-specific antibody, repeated freeze-thaw cycles may reduce affinity; aliquot and store at -20°C following initial use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material procurement, offering a streamlined one-stop solution from concept to clinic. This anti-Phospho-MAPKAPK2 (T334) antibody supports research in stress signaling, inflammation, and DNA damage pathways. Beyond single reagents, we supply validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary materials, and can assist in sourcing rare-target raw materials, ensuring you have the comprehensive support needed for diagnostic development and research continuity.