Anti-Phospho-MAPKAPK-2/MK2-T334 Rabbit Polyclonal Antibody for IHC-P, ELISA - P49137

Core Product Specifications and Parameters

Biological Background: MAPKAPK2 Function and Localization

• MAP kinase-activated protein kinase 2 (MAPKAPK2), also known as MK2, is a stress-activated serine/threonine-protein kinase that functions downstream of p38 MAP kinase in diverse cellular processes including cytokine production, endocytosis, cytoskeletal reorganization, and cell cycle control.
• Activated through phosphorylation by p38-alpha/MAPK14, primarily on threonine 334 (T334), MAPKAPK2 phosphorylates a wide range of substrates involved in post-transcriptional regulation of inflammatory mediators, heat shock protein function, and DNA damage response.
• Phosphorylates HSF1, inhibiting its transactivation activity by promoting interaction with HSP90 proteins and preventing homotrimerization and DNA binding.
• Related references: PMID:16278218
• Involved in cellular stress responses, including ultraviolet irradiation-induced signaling, where it phosphorylates CEP131 to regulate centriolar satellite formation via 14-3-3 protein binding.
• Related references: PMID:26616734
• Post-transcriptionally controls TNF and IL6 mRNA stability by phosphorylating ARE-binding proteins such as ZFP36/TTP, ELAVL1, HNRNPA0, and PABPC1, thereby modulating their affinity for AU-rich elements.
• Contributes to the DNA damage-induced G2/M checkpoint by relocalizing from the nucleus to the cytoplasm and phosphorylating HNRNPA0 and PARN to stabilize GADD45A mRNA.
• Localizes to both the cytoplasm and nucleus, dynamically shuttling between compartments in a signal-dependent manner.
• Ubiquitously expressed across tissues and belongs to the MAPKAP kinase subfamily, featuring ATP-binding and phosphoprotein properties, with alternative splicing reported.

Experimental Guidance and Technical Tips

• This antibody is phospho-specific for the T334 site; use phosphatase inhibitors during sample collection and lysis to preserve phosphorylation status.
• For IHC-P, include appropriate positive and negative tissue controls and consider antigen retrieval optimization; validate staining in relevant human, mouse, or rat tissues.
• In ELISA applications, employ both phospho-peptide and non-phospho-peptide controls to confirm specificity and avoid cross-reactivity with non-phosphorylated MAPKAPK2.
• Since MAPKAPK2 is activated by multiple stress stimuli, consider inducing phosphorylation prior to detection in experimental systems where baseline T334 phosphorylation is low.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by providing a comprehensive range of IVD raw materials, technical services, and consulting from concept to clinic. This anti-phospho-MAPKAPK2 antibody is part of our catalog tailored for stress kinase and inflammation research, and we offer validated antibody pairs, bulk ancillary reagents, and sourcing for rare-target raw materials. Whether you need custom monoclonal antibody development, optimized polyclonal antibodies, or assistance with assay integration, CamelBio serves as your reliable B2B partner to streamline your IVD production pipeline.