Anti-Phospho-MAPK14 (T180/Y182) / MAPK11 (T180/Y182) / MAPK13 (T180/Y182) / MAPK12 (T183/Y1845) Rabbit Polyclonal Antibody for WB, ELISA - Q15759/P53778/O15264/Q16539

Core Product Specifications and Parameters

Biological Background: MAPK11 Function and Localization

• MAPK11 (p38β) is a serine/threonine kinase and a member of the p38 MAPK family (also including MAPK14/p38α, MAPK12/p38γ, MAPK13/p38δ) that acts as a central signaling hub in response to stress and inflammatory cytokines. Related references: PMID:12452429, PMID:20626350, PMID:35857590

• Activated p38 MAPKs phosphorylate RPS6KA5/MSK1 and RPS6KA4/MSK2, which in turn phosphorylate transcription factors (CREB1, ATF1, NF-kappa-B, STAT1, STAT3) and histone H3/HMGN1, promoting chromatin remodeling and immediate-early gene induction. Related references: PMID:9687510

• p38 MAPKs also phosphorylate and activate MKNK1/MNK1 and MKNK2/MNK2, kinases that regulate protein synthesis initiation via EIF4E2 phosphorylation. Related references: PMID:11154262

• In the nucleus, p38 MAPKs directly phosphorylate multiple transcription factors including ATF1, ATF2, ATF6, ELK1, TP53/p53, MEF2C, and MEF2A, thereby modulating gene expression. Related references: PMID:10330143, PMID:15356147, PMID:9430721

• p38 MAPK signaling promotes histone H3 Ser-10 phosphorylation (H3S10ph) at the promoters of inflammatory genes such as IL6, IL8, and IL12B, facilitating NF-kappa-B recruitment and transcriptional activation. Related references: PMID:10330143, PMID:15356147, PMID:9430721

• MAPK11 specifically phosphorylates NLRP1 downstream of MAP3K20/ZAK in response to UV-B and ribosome collisions, leading to inflammasome activation and pyroptosis. Related references: PMID:35857590

• MAPK11 phosphorylates the histone methyltransferase DOT1L on multiple residues (Ser-834, Thr-900, Ser-902, Thr-984, Ser-1001, Ser-1009, Ser-1104), potentially linking stress signaling to epigenetic regulation. Related references: PMID:38270553

• MAPK11 localizes to both the cytoplasm and nucleus, and is expressed at highest levels in the brain and heart, with additional expression in placenta, lung, liver, skeletal muscle, kidney, and pancreas.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide spanning T180/Y182 of human MAPK14/MAPK11. Due to high homology among p38 family members in the activation loop, the antibody may cross-react with phosphorylated forms of MAPK12 and MAPK13. Validate specificity using relevant knockout or phospho-deficient controls.
• Suitable for Western blot (WB) and ELISA. In WB, expect bands around 38–42 kDa corresponding to phosphorylated MAPK14/p38α, MAPK11/p38β, MAPK12/p38γ, and MAPK13/p38δ under stimulated conditions.
• Use fresh samples with phosphatase inhibitor cocktails to preserve phosphorylation. Avoid repeated freeze-thaw cycles of the antibody and consider using a blocking buffer optimized for phospho-specific antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supplies diagnostic manufacturers, laboratories, and research institutes with validated IVD raw materials, including this phospho-specific p38 MAPK antibody for stress signaling and inflammation research. Our one-stop platform offers ready-to-use monoclonal and polyclonal antibodies, antibody pairs, bulk ancillary reagents, and bespoke sourcing for rare targets—ensuring seamless integration from concept to clinic. For large-volume requirements or customized conjugated formats, contact our B2B team to streamline your procurement and development workflow.