Anti-Phospho-MAP3K5 (S1033) Rabbit Polyclonal Antibody for WB and ELISA - Q99683

Core Product Specifications and Parameters

Biological Background: MAP3K5 (ASK1) Function and Localization

• MAP3K5, also known as ASK1 (Apoptosis Signal-regulating Kinase 1) or MEKK5, is a serine/threonine kinase that acts as a core component of the MAP kinase signal transduction pathway, mediating cellular responses to environmental changes and determining cell fate such as differentiation and survival.
• It plays a crucial role in the apoptosis signal transduction pathway through mitochondria-dependent caspase activation, linking stress signals to programmed cell death.
• MAP3K5 is activated by various stressors including oxidative stress and receptor-mediated inflammatory signals (e.g., TNF, LPS). Once activated, it phosphorylates and activates MAP2K4/SEK1, MAP2K3/MKK3, MAP2K6/MKK6, and MAP2K7/MKK7, which in turn activate the JNK and p38 MAPK pathways, controlling transcription factors like AP-1.
• It is required for the innate immune response, essential for host defense against a broad range of pathogens, highlighting its role in immunity.
• Subcellularly, MAP3K5 localizes to the cytoplasm and endoplasmic reticulum.
• Abundantly expressed in heart and pancreas.
• Key structural features include an ATP-binding domain, coiled-coil region, and phosphorylation. The protein is subject to methylation and ubiquitin-like conjugation, and it is involved in host-virus interactions.

Experimental Guidance and Technical Tips

• This phospho-specific antibody was raised against a synthetic phosphopeptide corresponding to residues surrounding S1033 of human MAP3K5. For Western blot, consider including a phosphatase-treated control to confirm phosphorylation-dependent detection.
• While validated for WB and ELISA by the supplier, optimal dilutions and conditions should be titrated in the user's specific experimental system.
• Given the protein's involvement in stress signaling, sample preparation under reduced oxidative stress may be critical to preserve the phosphorylation state.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and researchers from concept to clinic. For targets like MAP3K5 involved in stress and apoptosis signaling, we offer access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Partner with us to streamline your procurement and accelerate assay development.